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This kit contains sufficient reagents for one 96 well plate (96 tests)
This kit is specific for Capsase 3 (CASP-3), a member of the Caspase family (cysteine-aspartic acid protease) and when activated, is an essential factor in cell apoptosis. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid.
