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D0672

Sigma-Aldrich

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

NACRES:
NA.25

form

liquid

usage

200 uses (0.1 mg size)
40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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This Item
D9281D6293D9780
form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

usage

200 uses (0.1 mg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

General description

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Components

Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Preparation Note

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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