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D4138

Deoxyribonuclease II from porcine spleen

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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₪317.00

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About This Item

CAS Number:
9025-64-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
MFCD00130919
EC Number:
3.1.22.1 (BRENDA, IUBMB)

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Product Name

Deoxyribonuclease II from porcine spleen, Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

type

Type IV

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

foreign activity

RNase ≤0.05%

storage temp.

−20°C

Quality Level

200

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This Item
D8764D4513D4527
Deoxyribonuclease II from porcine spleen Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Sigma-Aldrich

D4138

Deoxyribonuclease II from porcine spleen

Deoxyribonuclease II from bovine spleen Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Sigma-Aldrich

D8764

Deoxyribonuclease II from bovine spleen

Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4513

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I from bovine pancreas Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4527

Deoxyribonuclease I from bovine pancreas

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

specific activity

≥1,000 units/mg protein

specific activity

≥2,000 units/mg protein

specific activity

≥2,000 units/mg protein

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

45 kDa

mol wt

38 kDa

mol wt

~31 kDa

mol wt

~31 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

300

foreign activity

RNase ≤0.05%

foreign activity

RNase ≤0.1%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

foreign activity

Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]
Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Other Notes

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM

Physical form

Contains sodium chloride

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000387972
0000364272
0000387972
0000364272
SLCL3793

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Yoko Kato-Toma et al.
The Biochemical journal, 371(Pt 1), 175-181 (2003-01-07)
13C-NMR spectroscopy was used to estimate the p K a values for the Tyr(150) (Y150) residue in wild-type and mutant class C beta-lactamases. The tyrosine residues of the wild-type and mutant lactamases were replaced with (13)C-labelled L-tyrosine ([ phenol -4-(13)C]tyrosine)
U Plöckinger et al.
European journal of endocrinology, 166(2), 223-234 (2011-11-09)
Somatostatin analogues (SSA) reduce autonomous GH secretion by activating somatostatin receptors (sst) 2 and 5 in 50-60% of acromegalic patients. However, by inhibiting insulin secretion these SSA reduce glucose tolerance. DG3173 is a novel SSA with additional binding to sst4
C C Wang et al.
The Journal of biological chemistry, 273(27), 17192-17198 (1998-06-27)
Porcine spleen DNase II, a lysosomal acid hydrolase, is a noncovalently linked alpha.beta heterodimer (Liao, T.-H. (1985) J. Biol. Chem. 260, 10708-10713). The alpha subunit, after disulfide cleavage, yields two chains, alpha1 and alpha2. The complete amino acid sequences of
F Chianini et al.
Veterinary immunology and immunopathology, 82(3-4), 245-255 (2001-10-06)
The distribution of different cells of the immune system has been studied in formalin-fixed paraffin-embedded tissues from conventionally reared healthy pigs, using immunohistological techniques. The samples collected were: lungs, tonsils, lymph nodes (mediastinal, mesenteric, inguinal and submandibular), pancreas, spleen, liver
T H Liao et al.
Biochimica et biophysica acta, 1007(1), 15-22 (1989-01-23)
Porcine spleen DNase II (EC 3.1.22.1), one of the best-characterized DNases II, is subcellularly located in lysosomes because the enzyme is co-sedimented with two of the lysosomal marker enzymes, cathepsin D and acid phosphatase. The physicochemical properties, including the subunit
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