Merck
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D6293

Sigma-Aldrich

pUC18 DNA Hae III Digest

for DNA electrophoresis

NACRES:
NA.25

form

liquid

usage

200 uses (0.1 mg size)
40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

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This Item
D9281D9780D0672
form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

usage

200 uses (0.1 mg size)

usage

100 uses (0.1 mg size), 500 uses (0.5 mg size)

usage

100 uses (0.1 mg size), 1000 uses (1 mg size), 20 uses (20 μg size), 500 uses (0.5 mg size)

usage

200 uses (0.1 mg size), 40 uses (20 μg size)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

suitability

suitable for electrophoresis (DNA)

Quality Level

100

Quality Level

100, 200

Quality Level

100, 200

Quality Level

100, 200

General description

Sigma′s pUC18 DNA Hae III Digest contains 11 fragments from 11 - 587 bp. About three ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
pUC18 DNA Hae III Digest has been used as a DNA marker to compare the internal control developed for the competitive polymerase chain reaction (PCR) for the detection of Butyrivibrio fibrisolvens in the rumen.

Components

Sigma′s pUC18 DNA Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 11 fragments, 11–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5%.

Preparation Note

Plasmid pUC18 DNA was digested with Hae III to completion, then purified by phenol extraction.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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