This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Promotion Available
2 of 6 reviewers received a sample product or took part in a promotion
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
Sign In to View Organizational & Contract Pricing.
Spend ₪1,600 — Save 5%; Spend ₪2,500 — Save 10%; Spend ₪4,250 — Save 15%*
Sign in to check eligibility. Shop Products
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
500 mL | Please contact Customer Service for Availability | ₪242.00 |
1 L | Please contact Customer Service for Availability | ₪441.00 |
6 x 500 mL | Please contact Customer Service for Availability | ₪1,221.00 |
24 x 500 mL | Please contact Customer Service for Availability | ₪4,648.00 |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Product Name
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
shipped in
ambient
storage temp.
2-8°C
General description
Listen to an Audio Overview
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
1 of 1
This Item | |||
|---|---|---|---|
| description With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture | description With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture | description liquid, sterile-filtered, with 4500 mg/L dextrose, with 4.0 mM L-glutamine, without sodium pyruvate, suitable for cell culture |
| form liquid | form powder | form powder | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility - | sterility - | sterility sterile-filtered |
| components HEPES: no | components glucose: high | components phenol red: yes | components glucose: high |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Still not finding the right product?
Explore all of our products under Dulbecco′s Modified Eagle′s Medium - high glucose
Storage Class
12 - Non Combustible Liquids
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
How much does D6429-1L weigh? Thank you!
1 answer-
Helpful?
-
-
Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 answer-
This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentationHelpful?
-
-
What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 answer-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
Helpful?
-
-
After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
1 answer-
Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
Helpful?
-
-
I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 answer-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
Helpful?
-