F4274

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

• Synonym(s): FITC, Fluorescein 5-isothiocyanate
• Empirical Formula (Hill Notation): C₂₁H₁₁NO₅S
• CAS Number: 3326-32-7
• Molecular Weight: 389.38
• NACRES: NA.47
• PubChem Substance ID: 24894860
• UNSPSC Code: 12171500
• EC Number: 222-042-0
• MDL number: MFCD00005063
• Beilstein/REAXYS Number: 1407295

Properties

• Product Name: Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• assay: ≥97.5% (HPLC)
• form: powder
• technique(s): titration: suitable
• color: yellow to very dark yellow
• mp: >360 °C (lit.)
• solubility: acetone: soluble 10 mg/mL
• fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: 2-8°C
• Quality Level: 100

Description

Application

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H317,H334
• pcodes: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves