Skip to Content
Merck
  • Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.

Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers.

Nature methods (2014-03-19)
Johan Hattne, Nathaniel Echols, Rosalie Tran, Jan Kern, Richard J Gildea, Aaron S Brewster, Roberto Alonso-Mori, Carina Glöckner, Julia Hellmich, Hartawan Laksmono, Raymond G Sierra, Benedikt Lassalle-Kaiser, Alyssa Lampe, Guangye Han, Sheraz Gul, Dörte DiFiore, Despina Milathianaki, Alan R Fry, Alan Miahnahri, William E White, Donald W Schafer, M Marvin Seibert, Jason E Koglin, Dimosthenis Sokaras, Tsu-Chien Weng, Jonas Sellberg, Matthew J Latimer, Pieter Glatzel, Petrus H Zwart, Ralf W Grosse-Kunstleve, Michael J Bogan, Marc Messerschmidt, Garth J Williams, Sébastien Boutet, Johannes Messinger, Athina Zouni, Junko Yano, Uwe Bergmann, Vittal K Yachandra, Paul D Adams, Nicholas K Sauter
PMID24633409
ABSTRACT

X-ray free-electron laser (XFEL) sources enable the use of crystallography to solve three-dimensional macromolecular structures under native conditions and without radiation damage. Results to date, however, have been limited by the challenge of deriving accurate Bragg intensities from a heterogeneous population of microcrystals, while at the same time modeling the X-ray spectrum and detector geometry. Here we present a computational approach designed to extract meaningful high-resolution signals from fewer diffraction measurements.

MATERIALS
Product Number
Brand
Product Description
578002
Sigma-Aldrich
Zinc, nanopowder, 40-60 nm avg. part. size, ≥99% trace metals basis
Pricing and availability is not currently available.
499552
Sigma-Aldrich
Zinc preparation, 5 g/dL Zn2+ in THF, highly reactive Rieke®metal
Pricing and availability is not currently available.
31653
Sigma-Aldrich
Zinc, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.9%, granular
Pricing and availability is not currently available.
266353
Sigma-Aldrich
Zinc, pieces, 2-14 mesh, 99.9% trace metals basis
Pricing and availability is not currently available.
402583
Sigma-Aldrich
Zinc, shot, <12 mm, 99.99% trace metals basis
Pricing and availability is not currently available.
267635
Sigma-Aldrich
Zinc, shot, 5 mm, 99.999% trace metals basis
Pricing and availability is not currently available.
349410
Sigma-Aldrich
Zinc, foil, thickness 1.0 mm, 99.99% trace metals basis
Pricing and availability is not currently available.
266361
Sigma-Aldrich
Zinc, sticks, diam. 7-10 mm, 99.97% trace metals basis
Pricing and availability is not currently available.
267619
Sigma-Aldrich
Zinc, foil, thickness 0.25 mm, 99.999% trace metals basis
Pricing and availability is not currently available.
243469
Sigma-Aldrich
Zinc, granular, 20-30 mesh, ACS reagent, ≥99.8%
Pricing and availability is not currently available.
209988
Sigma-Aldrich
Zinc, dust, <10 μm, ≥98%
Pricing and availability is not currently available.
GF37185532
Zinc, foil, 15mm disks, thickness 0.1mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF10281721
Zinc, foil, 8mm disks, thickness 0.01mm, 99.9%
Pricing and availability is not currently available.
GF36646768
Zinc, foil, 6mm disks, thickness 0.125mm, as rolled, 99.99+%
Pricing and availability is not currently available.
GF49751781
Zinc, foil, 5m coil, thickness 0.05mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF62845296
Zinc, foil, 50mm disks, thickness 1.0mm, as rolled, 99.99+%
Pricing and availability is not currently available.
GF61117602
Zinc, foil, 6mm disks, thickness 0.35mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF13942379
Zinc, foil, 1m coil, thickness 0.025mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF22060213
Zinc, foil, 10mm disks, thickness 0.35mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF35684534
Zinc, foil, 4mm disks, thickness 0.025mm, as rolled, 99.99+%
Pricing and availability is not currently available.
GF32323339
Zinc, foil, 6mm disks, thickness 1.0mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF57898760
Zinc, wire reel, 0.5m, diameter 1.0mm, 99.999%
Pricing and availability is not currently available.
GF22469137
Zinc, foil, 2m coil, thickness 0.35mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF32078853
Zinc, foil, light tested, 150x150mm, thickness 0.05mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF62468466
Zinc, foil, 2m coil, thickness 0.175mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF65881783
Zinc, foil, 4mm disks, thickness 0.35mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF39654984
Zinc, foil, 6mm disks, thickness 0.20mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF42897886
Zinc, foil, 8mm disks, thickness 0.15mm, as rolled, 99.95+%
Pricing and availability is not currently available.
GF21025314
Zinc, foil, not light tested, 50x50mm, thickness 0.015mm, 99.9%
Pricing and availability is not currently available.
GF37353225
Zinc, foil, 4mm disks, thickness 0.5mm, as rolled, 99.99+%
Pricing and availability is not currently available.