MABT400

Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody

clone 5C11, from mouse

• Synonym(s): Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 5C11, monoclonal
• species reactivity: canine, bovine, goat, rat, porcine, human, guinea pig
• technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001121180
• UniProt accession no.: Q92954
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PRG4(10216)

Description

General description

The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.

~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.

Immunogen

Recombinant protein corresponding to human Lubricin/Proteoglycan 4.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Western Blotting Analysis: A representative lot detected purified human synovial lubricin, as well as lubricin in synovial fluid recovered from patients with osteoarthritis (OA), camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), or from bovine, porcine, goat, dog, rat, and guinea pig (Ai, M., et al. (2015). PLoS One. 10(2):e0116237).
Western Blotting Analysis: A representative lot detected purified bovine synovial lubricin from calf and steer under both reduced and non-reduced conditions (Schmidt, T.A., et al. (2009). Biochim Biophys Acta. 1790(5):375-384).

This Anti-Lubricin/Proteoglycan 4, clone 5C11 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in Lubercin purified protein.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected 10 µg of purified Lubricin/Proteoglycan 4 protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable