If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
93362
Trizma® base
≥99.8% (T), crystalline, for molecular biology, BioUltra
Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
Select a Size
₹16,443.18
₹29,530.60
₹47,749.08
About This Item
Skip To
Product Name
Trizma® base, BioUltra, Molecular Biology, ≥99.8% (T)
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
SMILES string
NC(CO)(CO)CO
grade
Molecular Biology
description
aminopeptidase substrate
product line
BioUltra
assay
≥99.8% (T)
form
crystalline
impurities
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
ign. residue
≤0.01% (as SO4)
loss
≤0.5% loss on drying, 110 °C
color
colorless to white
pH
10.5-12.0 (25 °C, 4 M in H2O)
useful pH range
7-9
pKa (25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
168-172 °C
solubility
H2O: 4 M at 20 °C, clear, colorless
anion traces
chloride (Cl-): ≤20 mg/kg
sulfate (SO42-): ≤5 mg/kg
cation traces
Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
λ
4 M in H2O
UV absorption
λ: 260 nm Amax: 0.10
λ: 280 nm Amax: 0.08
suitability
in accordance for luminescence
Quality Level
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | T6791 | T6066 | RDD008 |
|---|---|---|---|
| grade for molecular biology | grade - | grade BioPerformance Certified | grade anhydrous |
| assay ≥99.8% (T) | assay ≥99.9% (titration) | assay ≥99.9% (titration) | assay ≥99.9% |
| product line BioUltra | product line BioXtra | product line - | product line Redi-Dri™ |
| Quality Level 200 | Quality Level 400 | Quality Level 400 | Quality Level 100 |
| form crystalline | form crystalline | form crystalline powder | form powder |
| pH 10.5-12.0 (25 °C, 4 M in H2O) | pH 10.5-12.0 (1 M in H2O) | pH 10.0-11.5 | pH 10.5-12, 7-9 |
Application
- In the dissection medium and culture medium of Hippocampal organotypic tissue cultures[1]
- As a component of lysis buffer before immunoprecipitation and immunoblotting[2]
- As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding[3]
- As a component of mixed buffer formulations[4][5]
- As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting[5]
General description
The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.
Other Notes
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.
Related Content
Trizma Base Specification Comparison
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service