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F4274

Fluorescein isothiocyanate isomer I

≥97.5% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein 5-isothiocyanate

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About This Item

Empirical Formula (Hill Notation):
C21H11NO5S
CAS Number:
3326-32-7
Molecular Weight:
389.38
NACRES:
NA.47
PubChem Substance ID:
24894860
UNSPSC Code:
12171500
EC Number:
222-042-0
MDL number:
MFCD00005063
Beilstein/REAXYS Number:
1407295

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Product Name

Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

assay

≥97.5% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to very dark yellow

mp

>360 °C (lit.)

solubility

acetone: soluble 10 mg/mL

fluorescence

λex 492 nm; λem 518 nm (green)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Quality Level

100

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Show Differences

1 of 4

This Item
F72504695134321-M
Fluorescein isothiocyanate isomer I ≥97.5% (HPLC)

Sigma-Aldrich

F4274

Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I suitable for protein labeling, ≥90% (HPLC), powder

Sigma-Aldrich

F7250

Fluorescein isothiocyanate isomer I

Fluorescein isothiocyanate isomer I ≥97.5% (HPLC)

Sigma-Aldrich

46951

Fluorescein isothiocyanate isomer I

FITC, Isomer I

Sigma-Aldrich

34321-M

FITC, Isomer I

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

technique(s)

-

fluorescence

λex 492 nm; λem 518 nm (green)

fluorescence

λex 492 nm; λem 518 nm (green)

fluorescence

λex 492 nm; λem 518 nm (green), λex 492 nm; λem 518 nm in 0.1 M phosphate pH 8.0

fluorescence

-

color

yellow to very dark yellow

color

orange to dark orange

color

-

color

orange-red

form

powder

form

powder

form

powder

form

solid

solubility

acetone: soluble 10 mg/mL

solubility

acetone: 1 mg/mL

solubility

acetone: soluble 0.1 g/10 mL

solubility

DMSO: 5 mg/mL, DMF: soluble, aqueous buffer, pH ≥ 9.0: soluble

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

Application

Fluorescein isothiocyanate isomer I has been used for the staining of conidia.[1][2]
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.[3][4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

related product

Product No.
Description
Pricing

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN1689
BCCH6569
BCBZ8362
BCBQ3411
BCBQ3411V

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Binding of live conidia of Aspergillus fumigatus activates in vitro-generated human Langerhans cells via a lectin of galactomannan specificity.
Persat F
Clinical and Experimental Immunology, 133, 370-370 (2003)
PU.1 serves a critical role in the innate defense against Aspergillus fumigatus via dendritic cell-associated C-type lectin receptor-1 and toll-like receptors-2 and 4 in THP-1-derived macrophages.
Liu C
Molecular Medicine Reports, 15, 4084-4084 (2017)
Ana Bratuša et al.
Colloids and surfaces. B, Biointerfaces, 181, 561-566 (2019-06-12)
This work describes the derivatization of dextran using N-(tert-butyloxycarbonyl)-S-(trityl)-L-cysteine in the presence of N,N'-carbonyldiimidazole (CDI) as a coupling agent. Homogeneous reactions in dimethyl sulfoxide allowed for an efficient coupling of the amino acid derivative to the polymer backbone. Derivatization was
Handbook of Affinity Chromatography (2005)
Hermanson GT
Bioconjugate Techniques (2013)
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