The presence of urea can reduce the depletion of albumin with PROTIA. We would suggest a buffer exchange prior to depletion using either dialysis or 5 KDa NMWL cut-off spin filters.
PROTIA
ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit
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1 KIT
₹1,20,501.60
About This Item
UNSPSC Code:
41106500
NACRES:
NA.56
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technique(s)
immunodepletion: suitable
storage temp.
2-8°C
1 of 4
This Item | PROTMEM | PROTDT | PROTBA |
|---|---|---|---|
| technique(s) immunodepletion: suitable | technique(s) - | technique(s) - | technique(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
ProteoPrep® Immunoaffinity Albumin and IgG Depletion Kit might be a useful tool for novel biomarker discovery.[1]
The ProteoPrep® Immunoaffinity medium in the prepacked spin columns is a mixture of two-beaded media containing recombinantly expressed, small single-chain antibody ligands, resulting in low non-specific binding and high capacity. The ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit has been designed to specifically remove albumin and IgG from human serum (25-50μL) in preparation of samples for proteomics analysis, two-dimensional electrophoresis (2DE) or liquid chromatography (LC). This kit is also effective in depleting albumin and IgG from mouse and guinea pig serum.
Application
Features and Benefits
- High Capacity removes more interfering abundant proteins
- High Specifity ensures you see an accurate and more complete proteome
- Buffer systems are compatible with 2 dimensional electrophoresis so there are no intermediate time consuming steps that might result in protein loss
Legal Information
ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
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Elena Guillén-Gómez et al.
Journal of diabetes research, 2018, 6165303-6165303 (2018-06-02)
Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome. We included type 2 diabetic normotensive non-obese males with (n
Carmen R M Bria et al.
Analytical and bioanalytical chemistry, 411(3), 777-786 (2018-11-25)
High- and low-density lipoproteins (HDL and LDL) are attractive targets for biomarker discovery. However, ultracentrifugation (UC), the current methodology of choice for isolating HDL and LDL, is tedious, requires large sample volume, results in sample loss, and does not readily
Yu Wang et al.
Molecular medicine reports, 16(5), 6276-6284 (2017-08-30)
Nephrotic syndrome is not a single disease; rather, it is a term for numerous diseases and pathological types. Renal biopsy is of use in determining the diagnosis and prognosis, and for guiding treatment; however, the use of this intervention is
Federica Re et al.
Journal of tissue engineering, 10, 2041731419845852-2041731419845852 (2019-05-21)
Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin-chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow
Andrea Bianchetti et al.
Frontiers in cell and developmental biology, 9, 650490-650490 (2021-06-01)
Human platelet lysate (hPL) is considered a valid substitute to fetal bovine serum (FBS) in the expansion of mesenchymal stromal cells (MSC), and it is commonly produced starting from intermediate side products of whole blood donations. Through freeze-thaw cycles, hPL
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My protein samples contain urea. Can I still use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, for depletion?
1 answer-
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Can I use the ProteoPrep® Immunoaffinity Albumin & IgG Depletion Kit, Product PROTIA, on samples besides serum?
1 answer-
Besides human serum or plasma samples, we have not specifically tested other human biological fluids with this kit. However, in principle, there is no reason not to try the kit with other fluid samples.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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