See the link below to review the formulations of the DPBS products. These concentrations are reported in grams per liter:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/269/385/d5652for.pdf
D5652
Dulbecco′s Phosphate Buffered Saline
Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture
Synonym(s):
DPBS
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10 L
€38.50
10 X 1 L
€66.40
50 L
€134.00
About This Item
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Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
pH
7.2-7.8
solubility
water: 9.6 g/L, clear
storage temp.
2-8°C
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1 of 4
This Item | D1283 | D8662 | D4031 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| form powder | form liquid | form liquid | form liquid |
| solubility water: 9.6 g/L, clear | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| pH 7.2-7.8 | pH - | pH - | pH - |
General description
Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.
Application
D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
- as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy[1]
- to dissolve Angelica gigas powder for microencapsulation[2]
- to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay[3]
- to wash amniotic membrane for cellular analyses[4]
Biochem/physiol Actions
Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Other Notes
This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Functional enhancement of ultrafine Angelica gigas powder by spray-drying microencapsulation
Choi KO, et al.
LWT--Food Science and Technology, 101, 161-166 (2019)
Ana C Bohórquez et al.
Journal of colloid and interface science, 506, 393-402 (2017-07-28)
The rotational diffusion of polyethylene glycol coated magnetic nanoparticles in serum albumin solutions was investigated in a range spanning 0mgmL
Iqbal Hyder et al.
BMC molecular and cell biology, 21(1), 9-9 (2020-03-01)
Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity.
Petr Heneberg et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 49(1), 54-67 (2018-10-06)
CD16 was previously suggested to be a new marker of basophils that is subject to downregulation by FcεRI crosslinking. Certain compounds, including supraoptimal concentrations of the PKC inhibitors, bisindolylmaleimides, decouple the release of granules containing CD203c, CD63 and histamine, and
Lauren E Prusinski Fernung et al.
The American journal of pathology, 188(10), 2293-2306 (2018-08-04)
Uterine fibroids (UFs) are clonal, hormonally regulated, benign smooth-muscle myometrial tumors that severely affect female reproductive health, although their unknown etiology limits effective care. UFs occur fourfold more commonly in African American women than in Caucasian women, and African American
Related Content
Instructions
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What is the formulation for this buffer? Molarity of Na2HPO4, KH2PO4, NaCl, KCl, etc?
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Is Product D5652, Dulbecco's Phosphate Buffered Saline, offered as a powder or a liquid?
1 answer-
Product No. D5652, Dulbecco's Phosphate Buffered Saline, is a powder.
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What is the pH of Product D5652, Dulbecco's Phosphate Buffered Saline?
1 answer-
The specification for the pH of D5652, Dulbecco's Phosphate Buffered Saline (when dissolved in water) is 7.2 to 7.8.
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Is Product D5652, Dulbecco's Phosphate Buffered Saline, a 1X solution once it has been diluted?
1 answer-
Product No. D5652, DPBS is a 1X solution after reconstitution at 9.6 g/L.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the sodium chloride concentration in Product D5652, Dulbecco's Phosphate Buffered Saline?
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This product contains 8.0 g/L sodium chloride.
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Can Product D5652, Dulbecco's Phosphate Buffered Saline, be autoclaved?
1 answer-
Product No. D5652, Dulbecco's Phosphate Buffered Saline should be autoclaved or sterile-filtered before use. It can be autoclaved at 121°C, 15 psi for 20 minutes.
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