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QuEChERS Method for Pesticide Residue Analysis

QuEChERS significantly improves laboratory efficiency and throughput. This procedure requires only small quantities of solvent and is capable of generating recoveries of 70-120% with RSDs <5% for a wide range of compounds.

QuEChERS Method for Pesticide Residue Analysis

A process in two steps:

1. Extraction

  • Solvent extraction techniques are designed to achieve maximum yield of analytes from the base matrix.
  • Solvent selection is important to minimize co-extracting compounds.
  • Analytes are extracted from the matrix with acetonitrile and salts/buffers.

2. Cleanup

  • Sample cleanup is necessary to reduce interferences.
  • Interferences can damage analytical instrumentation and complicate analyte identification and quantification.
  • The use of PSA, C18, GCB (Graphitized Carbon Black) or Z-Sep sorbents allow removal of sugars, lipids, sterols, organic acids, proteins, carotenoids, chlorophyll and other pigments prior to a GC-MS/MS or LC-MS/MS analysis.
  1. Homogenize sample
  2. Transfer sample to 50mL tube
  3. Add ACN and internal standard and shake for 1 min
  4. Extraction: Add extraction salts and centrifuge (55227-U for EN15662:2008 and 55234-U for 2007.01 AOAC)
  5. Cleanup: Transfer supernatant to cleanup tube
  6. Shake and centrifuge
    a) LC-MS/MS Analysis: Dilute extract with mobile phase
    b) GC-MS analysis: Analyze directly or after solvent exchange

QuEChERS Selection Guide

Products for the Extraction Step

Products for Cleanup (QuEChERS) Step

Method – EN15662:2008

Method – AOAC 2007.01

Specialty Products for Method EN15662 or AOAC 2007.01 – Alternatives