GE28-9909-44

Superdex^® 200 Increase 10/300 GL

Cytiva 28-9909-44, column L × I.D. 30 cm × 10 mm, 8.6 μm particle size

• Synonym(s): Superdex Increase
• UNSPSC Code: 41115700
• NACRES: SB.52

Properties

• material: glass column; polyethylene filter
• product line: Superdex^®
• form: prepacked column
• crosslinking: cross-linked
• mol wt: protein M_r 1.3 MDa (exclusion limit); protein M_r 10-600 kDa (fractionation range); M_p 1-100 kDa (dextrans fractionation range)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9909-44
• storage condition: (20% ethanol)
• parameter: 0.75 mL/min flow rate; 1.8 mL/min max. flow rate; 25-500 μL sample volume; 3.0 Mpa pressure drop required
• technique(s): liquid chromatography (LC): suitable
• bed volume: ~24 mL
• column L × I.D.: 30 cm × 10 mm
• matrix: cross-linked agarose-dextran composite
• matrix active group: dextran-agarose
• particle size: 8.6 μm
• cleaning: 1-14
• operating pH: 3—12
• separation technique: size exclusion (SEC)
• shipped in: ambient
• storage temp.: 2-30°C

Description

General description

Superdex^® 200 Increase size exclusion chromatography columns are well suited for high-resolution analysis and small-scale purification of monoclonal antibodies (mAb) and other biomolecules with Mr~ 10 000 to ~ 600 000.

Superdex™ 200 Increase is a next-generation, dextran-agarose composite matrix for SEC. With smaller and more rigid beads than their predecessors, Superdex™ Increase columns deliver higher resolution purification in shorter run times.

Superdex™ 200 Increase has a selectivity curve optimized for excellent resolution of antibodies and macromolecules within the range Mr ~ 100 000 to ~ 300 000. It is well suited for detection and separation of antibody monomers, dimers, and aggregates present in monoclonal antibody preparations. The outstanding resolving power of this resin facilitates protein characterization, including analysis of antibody size variants, assessment of membrane protein size homogeneity, and the study of protein-protein interactions.

Superdex™ 200 Increase is optimized to give high resolution separation for proteins with molecular weights in the range Mr ~ 100 000 to ~ 300 000. Superose™ 6 Increase provides a complementary fractionation range, resolving very large protein complexes and macromolecules that Superdex 200 Increase cannot separate.

This agarose-based resin is alkali-resistant and supports cleaning-in-place (CIP) procedures. This capability allows the same column to be used for different proteins, with minimal risk of carry-over between samples.

Application

For both preparative and analytical purposes

Gel filtration for small-scale (25-500 mL) preparative purification.

Long term use: all commonly used aqueous buffers, up to 8 M urea, ionic and non-ionic detergents, up to 6M guanidine hydrochloride

Features and Benefits

• Enhanced performance: improved resolution and runtime compared to predecessor Superdex™ 200.
• Very high resolution: small bead size and narrow particle size distribution provide high resolution, for high protein purity.
• High flow rates: rigid beads give excellent pressure/flow properties.

Preparation Note

Storage Temp.: 4°C to 30°C

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids