All Photos(2)

SCM139

Sigma-Aldrich

EZ-LiFT Stem Cell Passaging Reagent

Enzyme-free pluripotent stem cell dissociation solution, 100mL

eCl@ss:
32160801

form

liquid

application(s)

cell culture | stem cell: suitable

General description

A common problem associated with the routine culture of human pluripotent stem cells, including ES and iPS cells, is the unwanted spontaneous differentiation of cells. Without manual intervention, differentiated cells proliferate quickly and eventually diminish the overall quality of the pluripotent stem cell culture. Manual removal of differentiated cells is laborious and time consuming, making it difficult to process multiple hES/iPS cell lines simultaneously. Moreover, the risk for contamination is present each time the culture is subjected to this clean-up process.

EZ-LiFT Stem Cell Passaging Reagent is a proprietary enzyme-free and chemically defined stem cell dissociation reagent that selectively passages only undifferentiated pluripotent stem cells. The reagent eliminates the need for manual removal of differentiated cells and produces high cell viability without the need for ROCKi. By nature of its selectivity for undifferentiated pluripotent stem cells, EZ-LiFT can be used to rescue highly differentiated hES/iPS cultures, thus enabling the recovery of precious samples and time.

EZ-LiFT does not suffer from the drawbacks of other enzyme-based dissociation reagents such as Dispase, Collagenase IV and Accutase. The disadvantages of enzyme-based reagents are that they still require manual removal of differentiated cells, suffer from lot-to-lot variability and may carry the risk for karyotypic abnormalities.

Features and Benefits

• Enzyme-free and chemically defined formulation: Produces consistent results.
• Enriches for pluripotent stem cell populations: No manual clean-up required.
• Rescues highly differentiated hES/iPS culture: Saves precious samples and time.
• Gentle on cells with high cell survival rates: ROCKi is optional, but not required.

Application

Research Category
Cell Culture

Stem Cell Research

Quality

pH: 7.2-7.4
Sterility Tested: Pass
Osmolarity: 340-370 mOsm

Storage and Stability

EZ-LiFT Stem Cell Passaging Reagent can be stored at room temperature for 1 year without loss of activity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Protocols

Passaging Human iPS Cells Protocol Using EZ-LiFT™ Reagent

Human ips cell passaging protocol using EZ-LiFT™ Reagent, an enzyme-free and chemically defined pluripotent stem cell dissociation reagent.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service