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Merck

47140

4-Fluoro-7-nitrobenzofurazan

BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Synonym(s):

NBD-F

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About This Item

Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
29270-56-2
Molecular Weight:
183.10
UNSPSC Code:
12352100
NACRES:
NA.32
PubChem Substance ID:
329757296
MDL number:
MFCD00010196
Beilstein/REAXYS Number:
1077571

Key Documents

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Product Name

4-Fluoro-7-nitrobenzofurazan, BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

product line

BioReagent

assay

≥98.0% (HPLC)

mp

52-54 °C (lit.)

fluorescence

λex 470 nm; λem 528 nm in ethanol
λex 470 nm; λem 550 nm (Amino acid adducts)

suitability

suitable for fluorescence

storage temp.

2-8°C

Quality Level

200

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This Item
F588325455F3639
4-Fluoro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Sigma-Aldrich

47140

4-Fluoro-7-nitrobenzofurazan

4-Fluoro-7-nitrobenzofurazan ≥98% (elemental analysis)

Sigma-Aldrich

F5883

4-Fluoro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

25455

4-Chloro-7-nitrobenzofurazan

4-Fluoro-7-sulfamoylbenzofurazan

Sigma-Aldrich

F3639

4-Fluoro-7-sulfamoylbenzofurazan

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

suitability

-

fluorescence

λex 470 nm; λem 528 nm in ethanol, λex 470 nm; λem 550 nm (Amino acid adducts)

fluorescence

λex 380 nm; λem 515 nm (hydrolyzed (esterase))

fluorescence

λex 336 nm in methanol, λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)

fluorescence

-

assay

≥98.0% (HPLC)

assay

≥98% (elemental analysis)

assay

≥97.0% (HPLC)

assay

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

mp

52-54 °C (lit.)

mp

52-54 °C (lit.)

mp

97-100 °C, 97-99 °C (lit.)

mp

-

Application

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

235.4 °F - closed cup

flash_point_c

113 °C - closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF9196

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Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm
Chanda Ciriacks Klinker et al.
Analytical chemistry, 79(22), 8747-8754 (2007-10-13)
4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) has been characterized as a fluorogenic labeling reagent for high speed, online microdialysis-capillary electrophoresis (MD-CE) assays for amino acid neurotransmitters. NBD-F labeled amines are efficiently excited using 488 nm light allowing more common lasers to be used for
Bryan R Fonslow et al.
Analytical chemistry, 80(9), 3182-3189 (2008-03-21)
The continuous nature of micro free-flow electrophoresis (mu-FFE) was used to monitor the effect of a gradient of buffer conditions on the separation. This unique application has great potential for fast optimization of separation conditions and estimation of equilibrium constants.
Hongyi Zhang et al.
Analytical and bioanalytical chemistry, 386(5), 1387-1394 (2006-09-06)
An in-capillary derivatization of amino acids and peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed for their subsequent capillary electrophoretic analysis with laser-induced fluorescence detection (lambda (ex)=488 nm). The in-capillary derivatization was achieved in zone-passing mode by introducing successive plugs of sample
Hai Han et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(29), 3196-3202 (2011-03-05)
For a metabolomics study focusing on the analysis of aspartic and glutamic acid enantiomers, a fully automated two-dimensional HPLC system employing a microbore-ODS column and a narrowbore-enantioselective column was developed. By using this system, a detailed distribution of D-Asp and
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