This product is lyophilized from a solution containing 20 mM Tris, pH 8.0. For testing purposes, reconstitution is typically performed in the same buffer. The solubility of this product in water has not been validated. However, similar products are reported to be water soluble. Product SAE0102, lyophilized from PBS, pH 7.4, is soluble in water at 0.1 mg/mL. Product 169756, lyophilized from 100 mM sodium citrate buffer and 20 mM Tris-HCl, pH 8.3, is soluble in water on a lot-specific basis, approximately 1 mg/mL.
A2221
Antithrombin III from human plasma
lyophilized powder, ≥95% (SDS-PAGE)
Synonym(s):
Factor Xa inhibitor, Heparin cofactor
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25 μG
MXP 5,723.00
125 μG
MXP 18,030.00
About This Item
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biological source
human plasma
Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... SERPINC1(462)
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This Item | SRP6316 | A9024 | SAE0102 |
|---|---|---|---|
| assay ≥95% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay ≥70% protein basis (biuret) | assay ≥95% (SDS-PAGE) |
| biological source human plasma | biological source human | biological source human plasma | biological source - |
| Gene Information human ... SERPINC1(462) | Gene Information human ... SERPINC1(462) | Gene Information human ... ELA2(1991), SERPINA1(5265) | Gene Information - |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level - |
| form lyophilized powder | form lyophilized | form salt-free, lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
Biochem/physiol Actions
Serine protease inhibitor; Inhibitor of thrombin and activated factor X (Xa). Effective concentration equimolar with proteinase.
Physical form
Lyophilized from 20 mM Tris, pH 8.0
Other Notes
Not to be confused with thrombin antiserum.
One unit will form a complex and inhibit one unit of bovine thrombin at 25 degree C, using a 5 minute incubation in the presence of heparin.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Antithrombin activity is inhibited by acrolein and homocysteine thiolactone: Protection by cysteine.
Alejandro Gugliucci
Life sciences, 82(7-8), 413-418 (2008-01-22)
Conditions in which serum or tissue acrolein levels are high (e.g.: renal failure, heavy smoking, oxidative stress) are also associated with increased thrombogenicity. Another emerging cardiovascular risk factor is homocysteine, and its derivative, homocysteine thiolactone. Antithrombin is one of the
Yu-Fang Hsieh et al.
Biomedical materials (Bristol, England), 12(6), 065004-065004 (2017-10-06)
Biodegradable polymers have potential as a scaffold material for making small diameter artery bypass grafts. To resist thrombosis, maintain biocompatibility and enhance the remodeling of the grafts, it is crucial to modify polymer scaffolds so that the grafts have antithrombogenic
Xiaopeng Tang et al.
Cell research, 30(2), 119-132 (2019-12-08)
Coagulation balance is maintained through fine-tuned interactions among clotting factors, whose physiological concentrations vary substantially. In particular, the concentrations of coagulation proteases (pM to nM) are much lower than their natural inactivator antithrombin (AT, ~ 3 μM), suggesting the existence of other coordinators.
Hao Zhang et al.
Frontiers in cellular and infection microbiology, 11, 677902-677902 (2021-08-03)
Patients with sepsis may progress to acute respiratory distress syndrome (ARDS). Evidence of neutrophil extracellular traps (NETs) in sepsis-induced lung injury has been reported. However, the role of circulating NETs in the progression and thrombotic tendency of sepsis-induced lung injury
Protocols
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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If this product is lyophilized from 20mM Tris pH 8.0, the salts are already present in the lyophilized powder. So, to reconstitute this product only milliQ water should be added? Is there a specific volume to add?
1 answer-
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Would incubation of bovine thrombin with antithrombin in 1:1 ratio in the presence of heparin for 5 min at 25 degrees Celsius, form irreversible thrombin-antithrombin complex and prevent thrombin's ability to degrade Tos-Gly-Pro-Arg-ANBA-IPA substrate?
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This product forms an initial 1:1 complex to thrombin, followed by a slower rate of aggregation relative to bovine proteins. For assay purposes, in QC, the interaction of heparin, thrombin, and antithrombin III is allowed to proceed at 25°C for exactly two minutes and the residual thrombin activity in the reaction is measured for calculation. A brief search of the literature shows that ATIII is a serine protease inhibitor that irreversibly inactivates thrombin, a serine protease, in a 1:1 complex. It is recommended to test in this application to determine if there is residual thrombin activity.
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Dear, could you please provide me information what is the best way to dissolve product A2221 (Antithrombin III from human plasma)? Could I use distilled water? Would it be soluble at concentration 0.4 mg/ml? Thank you. Kind regards, Dusan
1 answer-
This product is lyophilized from a solution containing 20 mM Tris, pH 8.0. For testing purposes, the material is reconstituted in the same buffer. The solubility of this product in water has not been validated. However, similar available products are listed as being water soluble.
Product SAE0102, lyophilized from PBS, pH 7.4., soluble in water at 0.1 mg/mL
Product 169756, lyophilized from 100 mM sodium citrate buffer, 20 mM Tris-HCl, pH 8.3, soluble in water on a lot specific basis, approximately 1mg/mL
Product 1040504, USP reference standard lyophilized from 0.05 M Tris, 0.15 M NaCl, pH 7.4, 2 mg/mL of trehalose, and 10 mg/mL of human albumin. - water soluble, no rate givenHelpful?
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What is the molecular weight for Antithrombin III from human plasma. Product A2221?
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The molecular weight of Antithrombin III from human plasma is approximately 53 kDa.
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The Antithrombin III from human plasma, Product A2221, vial appears to be empty. What should I do?
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What you are observing is normal for this product. Each vial has sub-milligram amounts in it (either 25 or 125 micrograms), which may not be visible. One should reconstitute the vial, and measure the absorbance at 280 nm to show that it does have protein present.
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What is the specific activity of Antithrombin III from human plasma?
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We do not have strict specifications for the specific activity of the A2221. It is typically in the range of 40-80 units per mg protein. The value is reported on the certificate of analysis for each lot.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is Antithrombin III from human plasma, Product A2221, soluble in?
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The manufacturer has found it to be soluble in 50 mM Tris buffer with 150 mM sodium chloride. (No specific pH or concentration was provided.)
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