E9034
Phosphodiesterase 5A1 human
recombinant, expressed in Sf9 cells
Synonym(s):
3′,5′-cyclic-GMP phosphodiesterase, PDE 5A1
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recombinant
expressed in Sf9 cells
Quality Level
form
solution
specific activity
≥2,000 unit/μg protein
mol wt
126 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PDE5A(8654)
1 of 4
This Item | HPA004729 | HPA012873 | SRP0257 |
|---|---|---|---|
| specific activity ≥2,000 unit/μg protein | specific activity - | specific activity - | specific activity ≥1237 units/μg |
| Gene Information human ... PDE5A(8654) | Gene Information human ... PDE5A(8654) | Gene Information human ... PDE5A(8654) | Gene Information human ... PDE1A(5136) |
| recombinant expressed in Sf9 cells | recombinant - | recombinant - | recombinant expressed in baculovirus infected insect cells |
| form solution | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution | form aqueous solution |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
General description
N-terminal GST-tagged 126 kDa full-length protein
Research area: Cell SignallingPDE5is located in various tissues such as the kidney, brain, lungs, cardiac myocytes,vascular smooth muscle cells, penile corpus cavernosum, platelets, andgastrointestinal tissues.[1]
Biochem/physiol Actions
PDE5 regulates vascular smooth muscle contraction and is involved in NO-cGMP signaling in platelets to control aggregation. PDE5 may also be involved in the regulation of cGMP signaling in the brain and may modulate pressure-induced cardiac hypertrophy and fibrosis [2]. Phosphodiesterase (PDE)is an enzyme that is used to break phosphodiester bonds. Phosphodiesterase 5 (PDE5), amolecular target for the treatment of erectile dysfunction and pulmonaryhypertension. PDE5 is used to find novel PDE5 inhibitors. PDE5 specifically targets cyclicguanosine monophosphate (cGMP) generated by the activation of nitric oxide (NO). PDE5 regulates cGMP signalingin the cardiovascular and other tissues. At the genetic level PDE5 isexpressed as various isoforms and undergoes phosphorylation as a post-translationalmodification that results in its activation. PDE5 is degraded through theubiquitin pathway due to its nitrosylation.[1]
Physical form
Supplied as a solution in 20% glycerol containing 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% TWEEN® 20 and 3 mM DTT
Other Notes
One unit will convert 1.0 picomole of 3′,5′-cGMP to 5′-GMP per minute at pH 7.4 at 37 °C.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Wesam S Ahmed et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 134, 111128-111128 (2020-12-22)
Phosphodiesterase 5 (PDE5) is one of the most well-studied phosphodiesterases (PDEs) that specifically targets cGMP typically generated by nitric oxide (NO)-mediated activation of the soluble guanylyl cyclase. Given the crucial role of cGMP generated through the activation of this cellular
Andrew T Bender et al.
Pharmacological reviews, 58(3), 488-520 (2006-09-14)
Cyclic nucleotide phosphodiesterases (PDEs) are enzymes that regulate the cellular levels of the second messengers, cAMP and cGMP, by controlling their rates of degradation. There are 11 different PDE families, with each family typically having several different isoforms and splice
Felicia Akuamoa et al.
Toxicology in vitro : an international journal published in association with BIBRA, 73, 105130-105130 (2021-03-05)
Herbal supplements sold as 'all natural' on various markets in Accra (Ghana) and advertised as highly efficacious in treating erectile dysfunction (ED) were bought and analysed by a PDE-5 enzyme inhibition assay. The claimed efficacy of these products could be
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