Supelco® HPLC and UHPLC columns meet today’s demands of fast U/HPLC, LC-MS, and biopolymer separation, as well as regulated pharmacopeia and agency methods within various industries. Supelco® HPLC products are available with Fused-Core® particle technology, monolithic silica, and fully porous particles, including ultra-pure silica. In addition, porous graphitic carbon (PGC) particles, polymeric particles, alumina oxide, and zirconia particles are available for high pH stability.
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Superficially porous silica columns deliver higher speed and efficiency than fully porous particles of the same size. Fused-Core® HPLC columns like Ascentis® Express and BIOshell™ can convert any HPLC system into a fast HPLC workhorse with maximum speed and performance.
Monolithic silica-based Chromolith® HPLC columns allow rapid separations at very low column backpressure with high matrix tolerance and extended column lifetime, thus making them ideal products for high throughput and cost-efficient analyses of matrix rich samples. Chromolith® HPLC columns are made from monolithic silica with the bimodal pore structure having 2 µm macropores and 13 nm mesopores. This structure leads to various key advantages to the column.
Chromolith® WP column is a wide pore (300 Å) monolithic silica column made up of a single continuous rod of high purity, porous silica. This column has shown great potential compared to conventional columns with its consistent performance over a longer lifetime. In addition to various RP-phases a Protein A affinity column and an epoxy column, enabling customer specific modifications, are available.
We have a wide range of chiral HPLC columns for various applications.
This presentation focuses on recent advances, in the past two years, in 'carbon-HPLC' in the analysis of compounds classically associated with being difficult to separate by conventional 'silica-HPLC.'
Learn about porous graphitic carbon, a stationary phase material that is fully porous, carbon-based material—it is capable of analyzing small molecules and smaller proteins and peptides.
In this webcast, the origins and problems in LC–MS arising from impurities and contaminations will be described in detail and tips and tricks will be provided in order to enable or maintain high quality and high sensitivity LC-MS analyses.
Quantitation of monoclonal antibodies by LC-MS is a difficult analytical challenge due to the complexity in the workflow, from sample prep to LC-MS quantitation. This presentation will highlight a simple and high throughput method for the quantitation of monoclonal antibodies and proteins in human and animal serum.
*Tosoh Bioscience columns are available in the following countries:
Bosnia & Herzegovina
Central Afr. Rep
Congo dem. Rep.
United Kingdom (UK)