The Scepter™ cell counter uses the Coulter principle of impedance-based particle detection to reliably and accurately count every cell in your sample.
Start with a single-cell suspension, diluted to a total volume of 100 µL (recommended) in phosphate buffered saline (such as EmbryoMax® 1x DPBS) to 10,000-500,000 cells/mL (operating range) in a 1.5 mL microcentrifuge tube.
Turn on the Scepter™ cytometer by pressing the control button on the back of the instrument and wait for on-screen instructions to appear.
When prompted, attach a sensor to the end of the Scepter™ unit with the electrode sensing panel facing toward the front of the instrument, and you’ll see detailed instructions for each step of the counting process.
Pipette once to draw sample into the sensor. 50 uL of your cell suspension is drawn into the microfabricated, precision-engineered channel embedded in the sensor. The cell sensing zone detects each cell drawn into the sensor and thus cell concentration is calculated.
The sensing zone also measures cell sizes and cell volumes with sub-micron and sub-picoliter resolution, enabling the Scepter™ cytometer to display a histogram distribution of cell size or cell volume.
Figure 1. Counting Process
Figure 2. Cell Gating Process
Store up to 72 histograms on the Scepter™ cell counter itself, or upload data to your personal computer using the included software and USB cable. The Scepter™ Application Software shows uploaded volume and size distribution data as shown below. Easily share your data or conduct in-depth analysis by exporting uploaded files to Microsoft Excel.
Figure 3. Scepter™ 2.0 Software Pro User Interface