Thin layer chromatography (TLC), an analytical technique often used to separate and identify compounds present in a given mixture, can also be used to determine the purity of a particular substance within that mixture. TLC is ecomomical; hence it is often used for preliminary evaluations of separation parameters for column chromatography. Because of its simplicity and speed, TLC is also ideal for monitoring chemical reactions by qualitatively analyzing the reaction products. We offer a broad range of TLC products to address this wide variety of application needs. Here we describe various selection criteria that might be considered in choosing the ideal TLC plate for a particular application.
We offer a wide selection of standard analytical TLC plates. In addition to standard analytical TLC plates, Supelco offers a variety of both high performance and preparative TLC plates. High-performance Thin Layer Chromatography (HPTLC) plates are characterized by smaller particles, usually less than 10 µm, thinner layers, less than 200 µm and smaller plates with less than 10 cm developing distance. HPTLC offers distinct advantages over traditional TLC, which include better resolving power, faster development times, lower sample diffusion, and reduced solvent consumption. Like standard TLC plates, HPTLC plates are available coated with a variety of matrices and with or without indictors. Preparative TLC plates are used to purify or isolate a particular substance by separating it from contaminants. To allow them to separate larger quantities of material, these plates have adsorbent layer thicknesses of greater than 100 µm. They also typically have softer inorganic binders so sample bands can be removed more easily after separation.
Three types of backing are available for individual application requirements; glass, aluminum and plastic. Rigid, transparent and reusable, glass plates offer high chemical resistance and good heat stability for charring. For these reasons, they are the most widely used plate support. Aluminum foil plates are a support alternative that offers ease of handling and resistant to breakage. Lightweight and thinner than glass plates, aluminum plates can reduce shipping costs and save storage space. Additionally, they can be easily cut with scissors to the desired dimensions or stored in laboratory notebooks. Solvent resistance and compatibility with aqueous eluents are other features of aluminum plates which may be appealing for specific applications. Plastic or polyester (PET) plate supports are also lightweight and flexible. Like aluminum-backed plates, they can be cut to de
Our glass, aluminum foil and PET (polyester) TLC plates are available coated with a variety of matrices and are available with or without fluorescent indicator.
Available matrices include:
Plates are available with polymeric (organic) and inorganic binders. Binders do not generally impact the adsorptive properties of the matrix, but they do impact the adherence of the adsorbent layer to the backing.
Available binders include:
Choose from a comprehensive variety of plate dimensions: