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TRYPSEQM-RO

Roche

Trypsin Sequencing Grade, modified

from bovine pancreas

Synonym(s):
Trypsin
Enzyme Commission number:

Nivel de calidad

100

formulario

lyophilized (salt-free)

envase

pkg of 4 × 100 μg (11418033001)
pkg of 4 × 25 μg (11418025001)

manufacturer/tradename

Roche

pH óptimo

8.0

temp. de almacenamiento

2-8°C

Descripción general

Trypsin Sequencing Grade, modified, is isolated from bovine pancreas as a highly purified and specific protease, and subsequently modified.

Inhibitors:
TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin,α1-antitrypsin, APMSF, and antipain.

Especificidad

Trypsin is a serine endopeptidase. At pH 7.5–9, it specifically hydrolyzes proteins and peptide bonds C-terminally of Iysine and arginine. Amide and ester bonds of Arg and Lys are also cleaved. The specificity of Trypsin Sequencing Grade, modified, is verified with the oxidized B-chain of insulin (insulin Box) as a substrate. High concentrations of Trypsin Sequencing Grade, modified, one part by weight enzyme with 9 parts by weight insulin Box, are incubated for 18 hours to detect traces ofchymotrypsin impurities.

Aplicación

Use Trypsin Sequencing Grade, modified, to generate glycopeptides from purified glycoproteins.
It is used for:
  • Protein-structure elucidation
  • Tryptic mapping
  • Fingerprinting analysis
  • Sequence analysis
  • Translocation studies
  • Protein identification[1][2][3][4]

Calidad

Purity: Free of impurities that may interfere with the separation of peptides in reversed-phase HPLC.

Nota de preparación

Working concentration: 1/100 to 1/5 of the protein by weight
Storage conditions (working solution): -15 to -25 °C
Trypsin Sequencing Grade, modified, is more resistant to autolysis, even at pH values in the neutral and weakly basic range. The enzyme can be used in high concentrations.
A solution in 1% acetic acid or 1 mM HCI can be used for up to one week when stored at 2 to 8° C. Stored in aliquots at -15 to -25 °C, the solution is stable for at least one year without loss of activity.

Otras notas

For life science research only. Not for use in diagnostic procedures.

pictogramas

Exclamation markHealth hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificate of Analysis

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Certificate of Origin

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