Merck
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RTRYP-RO

Roche

Trypsin recombinant, Proteomics Grade

recombinant enzyme expressed in Pichia pastoris

Synonym(s):
Trypsin
Enzyme Commission number:

recombinante

expressed in Pichia pastoris

Nivel de calidad

100

ensayo

95% (gel filtration)

formulario

lyophilized

specific activity

≥150 units/mg protein

envase

pkg of 4 × 100 μg (03708969001)
pkg of 4 × 25 μg (03708985001)

manufacturer/tradename

Roche

temp. de almacenamiento

−20°C

Descripción general

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Especificidad

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Aplicación

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.[1][2]

Nota de preparación

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Reconstitución

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Otras notas

For life science research only. Not for use in diagnostic procedures.

pictogramas

Exclamation markHealth hazard

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

WGK

WGK 1

Punto de inflamabilidad F

does not flash

Punto de inflamabilidad C

does not flash

Certificate of Analysis

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Certificate of Origin

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