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CORET

Sigma-Aldrich

PCR Core kit with Taq DNA polymerase

PCR components in separate tubes to allow for optimization

eCl@ss:
32161000
NACRES:
NA.55

Nivel de calidad

200

formulario

liquid

uso

sufficient for 250 reactions

característica

dNTPs included
hotstart: no

technique(s)

PCR: suitable

color

colorless

input

purified DNA

enviado en

wet ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 °C without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.

Aplicación

CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.
PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.

Cantidad

Kit contains enough reagents for 250 units of Taq DNA polymerase.

Otras notas

The following reagents are required but not provided in the kit:
  • Mineral Oil, M 8662 (optional)
  • Thermocycler
  • Primers
  • DNA to be amplified
  • Chloroform, C 7559 (optional)

Información legal

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vial

  • P231710X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vial

  • M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vial

  • W1754Water, PCR Reagent 3 vial(s)

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

Código de clase de almacenamiento

10 - Combustible liquids

WGK

WGK 3

Punto de inflamabilidad F

Not applicable

Punto de inflamabilidad C

Not applicable

Certificado de Análisis

Certificado de origen

Genetic diversity and structure of the tree Manilkara zapota in a naturally fragmented tropical forest
Martiinez N, et al.
Journal of tropical medicine, 33(4), 285-294 (2017)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 14-14 (1989)

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico