Trajectory of DNA in the RNA polymerase II transcription preinitiation complex.

Proceedings of the National Academy of Sciences of the United States of America (1997-11-14)
T K Kim, T Lagrange, Y H Wang, J D Griffith, D Reinberg, R H Ebright

By using site-specific protein-DNA photocrosslinking, we define the positions of TATA-binding protein, transcription factor IIB, transcription factor IIF, and subunits of RNA polymerase II (RNAPII) relative to promoter DNA within the human transcription preinitiation complex. The results indicate that the interface between the largest and second-largest subunits of RNAPII forms an extended, approximately 240 A channel that interacts with promoter DNA both upstream and downstream of the transcription start. By using electron microscopy, we show that RNAPII compacts promoter DNA by the equivalent of approximately 50 bp. Together with the published structure of RNAPII, the results indicate that RNAPII wraps DNA around its surface and suggest a specific model for the trajectory of the wrapped DNA.

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RNA Polymerase II, p33 subunit human, recombinant, expressed in E. coli, ≥80% (SDS-PAGE)