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B6916

Supelco

Bradford Reagent

for 0.1-1.4 mg/ml protein

Synonym(s):

Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent

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About This Item

UNSPSC Code:
12161500
NACRES:
NA.32

Quality Level

form

solution

storage temp.

2-8°C

Related Categories

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.

Application

Bradford Reagent has been used to determine total protein concentration.

Features and Benefits

  • The reagent is ready to use. No mixing or dilution required.
  • Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
  • Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
  • Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
  • Can be used in microwell plate assays.
  • Inexpensive assay.

Legal Information

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Pictograms

Health hazardCorrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

Target Organs

Eyes,Central nervous system

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Articles

This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

Protocols

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Related Content

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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