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D3687

Sigma-Aldrich

DirectLoad PCR 100 bp Low Ladder

ready-to-use marker for DNA electrophoresis

Synonym(s):
100bp ladder for gel electrophoresis, 100bp maker, DirectLoad marker, RTU DNA ladder, RTU DNA marker, agarose gel electrophoresis ladder, agarose gel electrophoresis marker

grade

for molecular biology

Quality Level

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s DirectLoad PCR 100 bp Low Ladder contains 10 dsDNA fragments from 100 − 1,000 bases, evenly spaced in 100 bp repeats. Ladder should be loaded in a single lane on an agarose gel.

Application

Suitable for size determination of PCR generated DNA fragments with either agarose or polyacrylamide gels.

Features and Benefits

  • Ready-to-load
  • Easy-to-use
  • Popular band sizes

Components

Sigma′s DirectLoad PCR 100 bp Low Ladder is provided in a solution of 5% glycerol, 4.2 mM EDTA, 0.09% orange G, and 0.0125% xylene cyanol.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Legal Information

DirectLoad is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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