E0639

Endoglycosidase F2 from Elizabethkingia miricola

Name: Endoglycosidase F2 from <I>Elizabethkingia miricola</I>
Brand: SIGMA
Price: 3056 MYR

recombinant, expressed in E. coli, 20 U/mg

Synonym(s):

Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F2, Endo F2, Endoglycosidase F2 from Chryseobacterium meningosepticum, Endoglycosidase F2 from Elizabethkingia meningoseptica, Endoglycosidase F2 from Flavobacterium meningosepticum

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0.2 UNIT

RM 3,056.00

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About This Item

CAS Number:

37278-88-9

EC Number:

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00151069

UNSPSC Code:

12352204

NACRES:

NA.32

Key Documents

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recombinant

expressed in E. coli

Quality Level

200

conjugate

(N-linked)

form

solution

specific activity

20 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

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Show Differences

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This Item	E2264	E9762	P9120
Sigma-Aldrich E0639 Endoglycosidase F2 from Elizabethkingia miricola	Sigma-Aldrich E2264 Endoglycosidase F3 from Elizabethkingia miricola	Sigma-Aldrich E9762 Endoglycosidase F1 from Elizabethkingia miricola	Sigma-Aldrich P9120 PNGase F from Elizabethkingia meningoseptica
specific activity 20 U/mg	specific activity 30 U/mg	specific activity ≥16 U/mg	specific activity ≥10 units/mg protein
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
form solution	form solution	form buffered aqueous solution	form -
mol wt 32 kDa	mol wt 32 kDa	mol wt 32 kDa	mol wt 36 kDa
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

Packaging

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Physical form

Aseptically filled solution in 10 mM sodium acetate, 25 mM sodium chloride, pH 4.5

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000490687

0000446699

0000395651

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Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase.

Helena Ryšlavá et al.

The FEBS journal, 278(14), 2469-2484 (2011-05-14)

Fungal β-N-acetylhexosaminidases are inducible extracellular enzymes with many biotechnological applications. The enzyme from Penicillium oxalicum has unique enzymatic properties despite its close evolutionary relationship with other fungal hexosaminidases. It has high GalNAcase activity, tolerates substrates with the modified N-acyl group

An endo-β-N-acetylglucosaminidase from Enterococcus faecalis V583 responsible for the hydrolysis of high-mannose and hybrid-type N-linked glycans.

Liv Anette Bøhle et al.

FEMS microbiology letters, 325(2), 123-129 (2011-11-19)

It has been demonstrated previously that Enterococcus faecalis produces secreted endoglycosidases that enable the bacteria to remove N-linked glycans from glycoproteins. One enzyme potentially responsible for this activity is EF0114, comprising a typical GH18 endoglycosidase domain and a GH20 domain.

Remarkable transglycosylation activity of glycosynthase mutants of endo-D, an endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae.

Shu-Quan Fan et al.

The Journal of biological chemistry, 287(14), 11272-11281 (2012-02-10)

Endo-β-N-acetylglucosaminidase from Streptococcus pneumoniae (Endo-D) is an endoglycosidase capable of hydrolyzing the Fc N-glycan of intact IgG antibodies after sequential removal of the sialic acid, galactose, and internal GlcNAc residues in the N-glycan. Endo-D also possesses transglycosylation activity with sugar

Glycosylation of {beta}2 subunits regulates GABAA receptor biogenesis and channel gating.

Wen-Yi Lo et al.

The Journal of biological chemistry, 285(41), 31348-31361 (2010-07-20)

γ-aminobutyric acid type A (GABA(A)) receptors are heteropentameric glycoproteins. Based on consensus sequences, the GABA(A) receptor β2 subunit contains three potential N-linked glycosylation sites, Asn-32, Asn-104, and Asn-173. Homology modeling indicates that Asn-32 and Asn-104 are located before the α1

The N-glycan glycoprotein deglycosylation complex (Gpd) from Capnocytophaga canimorsus deglycosylates human IgG.

Francesco Renzi et al.

PLoS pathogens, 7(6), e1002118-e1002118 (2011-07-09)

C. canimorsus 5 has the capacity to grow at the expenses of glycan moieties from host cells N-glycoproteins. Here, we show that C. canimorsus 5 also has the capacity to deglycosylate human IgG and we analyze the deglycosylation mechanism. We

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Endoglycosidase F2 from Elizabethkingia miricola