E2264

Endoglycosidase F3 from Elizabethkingia miricola

Name: Endoglycosidase F3 from <I>Elizabethkingia miricola</I>
Brand: SIGMA
Price: 3982 MYR

recombinant, expressed in E. coli, 30 U/mg

Synonym(s):

Elizabethkingia miricola, Endo-β-N-acetylglucosaminidase F3, Endoglycosidase F3 from Elizabethkingia (Chryseobacterium/Flavobacterium) meningosepticum

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0.2 UNIT

RM 3,982.00

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.2.1.96 (BRENDA, IUBMB)

MDL number:

MFCD00146843

Key Documents

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Product Name

Endoglycosidase F3 from Elizabethkingia miricola, recombinant, expressed in E. coli, 30 U/mg

recombinant

expressed in E. coli

conjugate

(N-linked)

form

solution

specific activity

30 U/mg

mol wt

32 kDa

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

Related Categories

Glycobiology Profiling Tools

Proteins & Enzymes

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Show Differences

1 of 4

This Item	E0639	E9762	P9120
Sigma-Aldrich E2264 Endoglycosidase F3 from Elizabethkingia miricola	Sigma-Aldrich E0639 Endoglycosidase F2 from Elizabethkingia miricola	Sigma-Aldrich E9762 Endoglycosidase F1 from Elizabethkingia miricola	Sigma-Aldrich P9120 PNGase F from Elizabethkingia meningoseptica
specific activity 30 U/mg	specific activity 20 U/mg	specific activity ≥16 U/mg	specific activity ≥10 units/mg protein
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli
form solution	form solution	form buffered aqueous solution	form -
mol wt 32 kDa	mol wt 32 kDa	mol wt 32 kDa	mol wt 36 kDa
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

Application

Endoglycosidase F3 from Elizabethkingia miricola has been used to analyze core fucosylation and tryptic digests of serum proteins.[1]

Biochem/physiol Actions

Cleaves asparagine-linked biantennary and triantennary complex, oligosaccharides depending on the state of core fucosylation and peptide linkage.

Endoglycosidase F3 belongs to the glycoside hydrolase family 18 (GH18).[2] It has hydrolytic activity.[3] Endoglycosidase F3 glycosylates α-1,6-fucosylated GlcNAc derivative to give natural, core fucosylated complex-type N-glycopeptides.[4]

Other Notes

One unit will release N-linked oligosaccharides from 1 μmole of denatured porcine fibrinogen in 1 minute at 37 °C, pH 4.5.

Packaging

Supplied with 5× Reaction Buffer, 250 mM sodium acetate, pH 4.5

Physical form

Aseptically filled solution in 20 mM Tris-HCl, pH 7.5

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Advances in Carbohydrate Chemistry (2016)

Chemical Biology of Glycoproteins (2017)

Characterization of core fucosylation via sequential enzymatic treatments of intact glycopeptides and mass spectrometry analysis.

Liwei Cao et al.

Nature communications, 13(1), 3910-3910 (2022-07-08)

Core fucosylation of N-linked glycoproteins has been linked to the functions of glycoproteins in physiological and pathological processes. However, quantitative characterization of core fucosylation remains challenging due to the complexity and heterogeneity of N-linked glycosylation. Here we report a mass

Quantitative analysis of core fucosylation of serum proteins in liver diseases by LC-MS-MRM

Ma J, et al.

Journal of proteomics, 189, 67-74 (2018)

Characterization of novel endo-beta-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG

Huang Y, et al.

Scientific reports, 8(1), 246-246 (2018)

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