ABE542
Anti-YTHDF2 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
YTH domain-containing family protein 2, CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-YTHDF2 Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... YTHDF2(51441)
Analysis Note
Evaluated by Western Blotting in C6 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected YTHDF2 in 10 µg of C6 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected YTHDF2 in 10 µg of C6 cell lysate.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-YTHDF2 antibody is validated for use in WB, IC for the detection of YTHDF2.
Western Blotting Analysis: 15 µg/mL from a representative lot detected YTHDF2 in WT HeLa cell lysate and YTHDF2 overexpressing lysate (Chuan He, Univerity of Chicago).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected YTHDF2 in HeLa cells (Chuan He, Univerity of Chicago).
Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected YTHDF2 in HeLa cells (Chuan He, Univerity of Chicago).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
YTH domain-containing family protein 2 (YTHDF2), also known as CLL-associated antigen KW-14, High-glucose-regulated protein 8, Renal carcinoma antigen NY-REN-2, is also encoded by the gene name YTHDF2 and HGRG8. YTHDF2 recognizes specifically and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs. Recent studies have shown that YTHDF2 may play an important role in the efficiency of mRNA splicing, processing and stability. Additionally, YTHDF2 has been shown to act as a regulator of mRNA stability. And in the process of binding to m6A-containing mRNAs, YTHDF2 has been implicated to localize to mRNA decay sites, such as processing bodies (P-bodies), which leads to mRNA degradation.
~62 kDa observed. Uncharacterized band(s) may appear in some lysates.
Immunogen
Epitope: Localization to mRNA processing bodies (P-bodies)
KLH-conjugated linear peptide corresponding to the localization of mRNA processing bodies (P-bodies) to human YTHDF2.
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Wuxun Lu et al.
The Journal of biological chemistry, 293(34), 12992-13005 (2018-07-07)
The internal N6-methyladenosine (m6A) modification of cellular mRNA regulates post-transcriptional gene expression. The YTH domain family proteins (YTHDF1-3 or Y1-3) bind to m6A-modified cellular mRNAs and modulate their metabolism and processing, thereby affecting cellular protein translation. We previously reported that
Sarah N'Da Konan et al.
Retrovirology, 19(1), 4-4 (2022-02-02)
The recent discovery of the role of m6A methylation in the regulation of HIV-1 replication unveiled a novel layer of regulation for HIV gene expression. This epitranscriptomic modification of HIV-1 RNAs is under the dynamic control of specific writers and
Angelica Benavides-Serrato et al.
Cancer letters, 562, 216178-216178 (2023-04-16)
A major mechanism conferring resistance to mTOR inhibitors is activation of a salvage pathway stimulating internal ribosome entry site (IRES)-mediated mRNA translation, driving the synthesis of proteins promoting resistance of glioblastoma (GBM). Previously, we found this pathway is stimulated by
Xinxia Wang et al.
International journal of obesity (2005), 42(11), 1912-1924 (2018-03-01)
N6-methyladenosine (m6A) modification of mRNA plays an important role in regulating adipogenesis. However, its underlying mechanism remains largely unknown. Using Jinhua and Landrace pigs as fat and lean models, we presented a comprehensive transcriptome-wide m6A profiling in adipose tissues from
Ruifan Wu et al.
Cell death & disease, 10(3), 171-171 (2019-02-23)
Embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine, disease treatment, and organ transplantation. As the ethical issue of human ESCs and similarity of pig in human genome and physiological characteristics, the porcine
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