Skip to Content

Key Documents

View All Documentation

RTRYP-RO

Roche

Trypsin recombinant, Proteomics Grade

recombinant enzyme expressed in Pichia pastoris

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

EC Number:
3.4.21.4 (BRENDA, IUBMB)
NACRES:
NA.21
UNSPSC Code:
12352204
Specific activity:
≥150 units/mg protein
Assay:
95% (gel filtration)
Recombinant:
expressed in Pichia pastoris
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

recombinant

expressed in Pichia pastoris

assay

95% (gel filtration)

form

lyophilized

specific activity

≥150 units/mg protein

packaging

pkg of 4 × 100 μg (03708969001), pkg of 4 × 25 μg (03708985001)

manufacturer/tradename

Roche

storage temp.

−20°C

General description

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Application

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Biochem/physiol Actions

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Preparation Note

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!
Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Signal Word

Danger

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library