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Phosphoglucose Isomerase (PGI)

Name: Phosphoglucose Isomerase (PGI)
Brand: ROCHE

from yeast

Synonym(s):

glucose-6-phosphate isomerase

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About This Item

UNSPSC Code:

12352204

EC Number:

5.3.1.9 (BRENDA, IUBMB)

NACRES:

NA.54

eCl@ss:

32039090

Specific activity:

~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)

Biological source:

yeast

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Properties

biological source

yeast

form

suspension

specific activity

~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)

packaging

pkg of 1 mL (10127396001 [2 mg]), pkg of 1 mL (10128139001 [10 mg])

manufacturer/tradename

Roche

optimum pH

7.0-7.6

storage temp.

2-8°C

Description

General description

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.

Application

Isomerization of ketoses to aldoses.

Biochem/physiol Actions

Phosphoglucose isomerase (PGI) is specific for G6P (K_m = 0.7 mM) and fructose-6-phosphate (F6P).

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Analysis Note

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

Other Notes

For life science research only. Not for use in diagnostic procedures.

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This Item	P5442	P9544	P5538
Roche PGIRO Phosphoglucose Isomerase (PGI)	P5442 Active Recombinant Human Phosphoglucose Isomerase	Sigma-Aldrich P9544 Phosphoglucose Isomerase from rabbit muscle	Sigma-Aldrich P5538 Phosphoglucose Isomerase from Bacillus stearothermophilus
description from yeast	description Lyophilized powder, ≥ 100U/mg protein	description Type XI, lyophilized powder, ≥200 units/mg protein	description lyophilized powder, 300-1,000 units/mg protein
specific activity ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)	specific activity ≥100 U/mg Protein, ≥50 U/mg Solid	specific activity ≥200 units/mg protein	specific activity 300-1,000 units/mg protein
biological source yeast	biological source human	biological source rabbit muscle	biological source -
form suspension	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. -10 to -25°C	storage temp. −20°C	storage temp. −20°C
optimum pH 7.0-7.6	optimum pH -	optimum pH -	optimum pH -
manufacturer/tradename Roche	manufacturer/tradename -	manufacturer/tradename -	manufacturer/tradename -

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

No data available

Flash Point (°C)

No data available

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