This product has been used in influenza infection experiments as a supplement in the infection medium. The medium used is DMEM serum-free medium supplemented with 50 IU/ml penicillin, 50 mg/ml streptomycin, and 0.2 mg/ml TPCK-trypsin. Relevant references include:
"Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy," PLoS Pathogens (2021-02-18), PMID: 33596274, Irene Pila-Castellanos, Diana Molino, Joe McKellar, et al.
"Viruses traverse the human proteome through peptide interfaces that can be biomimetically leveraged for drug discovery," L Meyniel-Schicklin, J Amaudrut… - Proceedings of the National Academy of Sciences, 2024.
4352157
Trypsin, TPCK-Treated
Synonym(s):
6- (1-tosylamido-2-phenyl) ethyl chloromethyl ketone treated Trypsin, Modified trypsin (TPCK-Treated), Trypsin, TPCK treated
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.56
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shipped in
dry ice
storage temp.
−20°C
General description
Trypsin is a serine protease that specifically hydrolyzes peptide bonds at the carboxyl side of Lysine and Arginine residues. This modified trypsin has been treated with N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) to inactivate extraneous chymotryptic activity. This trypsin product also contains calcium chloride, which may improve consistency in digestion. Each package contains 10 vials, with 25 μg in each vial.
Application
Trypsin, TPCK-Treated has been used for the digestion of protein samples to generate peptides for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.[1][2][3]
Trypsin, TPCK-Treated has been used in enzymatic treatment to cleave the recombinant native B-cell lymphoma-extra large (Bcl-xL)-ΔTM monomer to generate fibrils (the third type of Bcl-xL fibril, called “BclxLcf37”).[4] It has also been used in influenza infection experiments as a supplement in infection/DMEM serum-free medium to infect A549 cells with influenza A H1N1 virus at a multiplicity of infection (MOI) to perform multi-round infection.[5]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Pavel Bouchal et al.
Cell reports, 28(3), 832-843 (2019-07-18)
Accurate classification of breast tumors is vital for patient management decisions and enables more precise cancer treatment. Here, we present a quantitative proteotyping approach based on sequential windowed acquisition of all theoretical fragment ion spectra (SWATH) mass spectrometry and establish
Quantitative milk proteomics--host responses to lipopolysaccharide-mediated inflammation of bovine mammary gland.
Danielsen M, et al.
Proteomics, 10, 2240-2249 (2010)
Alexis Gonneaud et al.
International journal of molecular sciences, 21(20) (2020-10-21)
Bcl-xL, a member of the Bcl-2 family, is a pro-survival protein involved in apoptosis regulation. We have previously reported the ability of Bcl-xL to form various types of fibers, from native to amyloid conformations. Here, we have mimicked the effect
Irene Pila-Castellanos et al.
PLoS pathogens, 17(2), e1009340-e1009340 (2021-02-18)
Influenza virus infections are major public health threats due to their high rates of morbidity and mortality. Upon influenza virus entry, host cells experience modifications of endomembranes, including those used for virus trafficking and replication. Here we report that influenza
Douglas E Vetter et al.
Methods in molecular biology (Clifton, N.J.), 493, 345-366 (2008-10-08)
Modern biologists have at their disposal a large array of techniques used to assess the existence and relative or absolute quantity of any molecule of interest in a sample. However, implementing most of these procedures can be a daunting task
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What is the most suitable buffer to reconstitute it considering a protocol to activate influenza virus prior to mammalian culture infection?
1 answer-
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Hi, We wonder how is the best buffer or solution to resuspend this product?. Cat 352157 Sigma-Aldrich. Trypsin, TPCK-Treated
1 answer-
For the purpose of in-gel tryptic digestion, it is recommended to reconstitute a vial of trypsin with 1 mL of 100 mM ammonium bicarbonate (NH4HCO3) pH 8.5.
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