This product has not been tested specifically for use in cell staining on slides or coverslips. However, it has been used successfully in staining osteoblasts by other customers. Cell are fixed prior to staining. Suitability for use with a specific cell type or for fresh smears would have to be determined by the end user. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/390/b1911dat-ms.pdf
B1911
BCIP®/NBT
alkaline phosphatase substrate, chromogenic, liquid
Synonym(s):
5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
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Product Name
BCIP®/NBT Liquid Substrate System, ready to use solution
form
liquid
storage temp.
2-8°C
Quality Level
Related Categories
1 of 4
This Item | B3679 | B3804 | 72091 |
|---|---|---|---|
| Quality Level 300 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Application
A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.
BCIP®/NBT Liquid Substrate System has been used:
- for staining alkaline phosphatase(ALP) in bone marrow stromal cells[1]
- as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody[2]
- as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays[3]
Disclaimer
It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Jungho Jang et al.
Scientific reports, 8(1), 7211-7211 (2018-05-10)
The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified
Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Ling M, et al.
Cell & Bioscience, 7(1), 27-27 (2017)
Salma E Jiménez-Badillo et al.
Scientific reports, 7(1), 13351-13351 (2017-10-19)
The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either
The evaluation of angiogenesis and matrix metalloproteinase-2 secretion in bone marrow of multiple myeloma patients before and after the treatment
Bolkun L, et al.
Advances in Medical Sciences, 58(1), 118-125 (2013)
Commensal microbes induce serum IgA responses that protect against polymicrobial sepsis
Wilmore JR, et al.
Cell host & microbe, 23(3), 302-311 (2018)
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Protocols
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
Related Content
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can the product be used for citological procedures? specifically, air dry citological slides?
1 answer-
Helpful?
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Should this be applied to fixed cells or live cells for staining?
1 answer-
Product B1911 is not formulated as a balanced salt solution. Therefore, it should be applied to fixed cells, as live cells are not suitable for staining with this solution.
Helpful?
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Is the BCIP in your BCIP/NBT Liquid Substrate part number B1911 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt?
1 answer-
The BCIP used in Product B1911 is not 5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt (CAS# 6578-06-9).
Helpful?
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