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HT501128

Formalin solution, neutral buffered, 10%

histological tissue fixative

Formalin solution, neutral buffered, 10%

Synonym(s):

Formaldehyde, Methanal

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About This Item

MDL number:
MFCD00003274
UNSPSC Code:
12352114
PubChem Substance ID:
24895887
NACRES:
NA.47

Key Documents

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biological source

synthetic

Quality Level

200

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

pH

6.90-7.10

density

1.080 g/cm3

application(s)

hematology
histology

storage temp.

room temp

SMILES string

[O-]#[C+H2]

InChI

1S/CH2O/c1-2/h1H2

InChI key

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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1 of 4

This Item
HT5011HT501320HT5012
Formalin solution, neutral buffered, 10% histological tissue fixative

Sigma-Aldrich

HT501128

Formalin solution, neutral buffered, 10%

Formalin solution, neutral buffered, 10% case of 48 × 15 mL, histological tissue fixative

Sigma-Aldrich

HT5011

Formalin solution, neutral buffered, 10%

Formalin solution, neutral buffered, 10% histological tissue fixative

Sigma-Aldrich

HT501320

Formalin solution, neutral buffered, 10%

Formalin solution, neutral buffered, 10% case of 24 × 60 mL, histological tissue fixative

Sigma-Aldrich

HT5012

Formalin solution, neutral buffered, 10%

biological source

synthetic

biological source

-

biological source

synthetic

biological source

synthetic

concentration

10% formalin (approx. 4% formaldehyde)

concentration

10% formalin (approx. 4% formaldehyde)

concentration

10% formalin (approx. 4% formaldehyde)

concentration

10% formalin (approx. 4% formaldehyde)

color

colorless

color

colorless

color

colorless

color

colorless

pH

6.90-7.10

pH

6.90-7.10

pH

6.90-7.10

pH

6.90-7.10

density

1.080 g/cm3

density

1.080 g/cm3

density

1.080 g/cm3

density

1.080 g/cm3

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative.[1] 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.[2] Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Application

Formalin solution, neutral buffered, 10% has been used as a popular general purpose histological fixative to fix tissues.[3][4][5]
Formalin solution, neutral buffered, 10% has been used in oil-red-o staining for the differentiation of 3T3-L1 preadipocyte cell line and mouse embryonic fibroblasts into adipocytes.[6] It has been used in the histological analysis of rabbit′s eye and diabetic rat intestine.[7][8]
Popular general purpose histological fixative.

Physical form

Neutral buffered solution

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCX1919
MKCW9484
MKCS9056
MKCP2042
MKCW0485

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Paul J Marchand et al.
Biomedical optics express, 8(7), 3343-3359 (2017-07-19)
We present a novel extended-focus optical coherence microscope (OCM) attaining 0.7 μm axial and 0.4 μm lateral resolution maintained over a depth of 40 μm, while preserving the advantages of Fourier domain OCM. Our system uses an ultra-broad spectrum from
Ablation of the transcriptional regulator Id1 enhances energy expenditure, increases insulin sensitivity, and protects against age and diet induced insulin resistance, and hepatosteatosis
Satyanarayana A, et al.
Faseb Journal, 26(1), 309-323 (2012)
Jonathan C J Wei et al.
Scientific reports, 7(1), 15885-15885 (2017-11-23)
Emerging micro-scale medical devices are showing promise, whether in delivering drugs or extracting diagnostic biomarkers from skin. In progressing these devices through animal models towards clinical products, understanding the mechanical properties and skin tissue structure with which they interact will
Effects of the marine microalgae Isochrysis galbana and Nannochloropsis oculata in diabetic rats
Nu?o K, et al.
Journal of functional foods, 5(1) (2013)
Comparison of partially and fully chemically-modified siRNA in conjugate-mediated delivery in vivo.
Hassler M R, et al.
Nucleic Acids Research, 46(5), 2185-2196 (2018)
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Product Information Sheet - HT501128

Product Information Sheet

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Questions

1–7 of 7 Questions  

  1. Does HT501128 contain sodium phosphate monobasic or sodium phosphate dibasic? If so, what is the final % W/V? Also what is the pH of HT501128 at 25C? Thank you

    1 answer

    1. The exact buffer concentration for HT511128 is not stated on the website and is considered confidential. The classic textbook formulation for 10% Neutral Buffered Formalin is as follows:

      100 ml Formaldehyde, 37-40%
      900 ml distilled water
      4.0 grams sodium phosphate, monobasic
      6.5 grams sodium phosphate, dibasic (anhydrous)

      The Specification Sheet states the pH range must be between 6.90 to 7.10. The temperature at which the pH is not measured but should be either 20° or 25°C.

      Helpful?

  2. Is the formalin sensitive to light?

    1 answer

    1. Neutral buffered formalin solutions are particularly sensitive to ambient light. Long term exposure to UV light may result in degradation. This product is packaged in natural opaque plastic. Note that solutions should be tightly closed during storage.

      Helpful?

  3. Does Formalin solution, neutral buffered, contain any alcohol?

    1 answer

    1. Yes, this product contains Methanol which is included in the formaldehyde starting material. The concentration of methanol in this product is 1.0 - 1.5%.

      Helpful?

  4. Can the product HT501128-4L, Formalin solution, neutral buffered, 10%, be used for immunofluorescence staining?

    1 answer

    1. It is generally expected that if tissues are left in 10% Neutral Buffered Formalin for extended periods, the tissue may not be suitable for immunofluorescence. However, if the tissue spends only a short time in the 10% NBF, it might still be usable for immunofluorescence.

      Helpful?

  5. Is this product HT501128-4L is RNase/nuclease free?

    1 answer

    1. Currently, none of the formalin solutions are nuclease free. Products that are exo or endo nuclease free are typically labeled as suitable for "molecular" use. If no testing is shown as being performed, the products are not claimed to be nuclease free.

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  6. Is there a 10% neutralized buffered formalin stating the osmolality?

    1 answer

    1. None of the formalin solutions are tested for osmolality. Osmolality is significant for live cells, as cells struggle to survive when their surroundings are vastly different from their own, on the other hand formalin is only a fixative solution. Formaldehyde solutions are buffered to prevent the formation of formalin pigment or acid hematin that forms when tissues are fixed at low pH.

      Helpful?

  7. What are the differences between products 1004960700 and HT501128?

    1 answer

    1. Products 1004960700 (Formaldehyde solution, 4% buffered) and HT501128 (10% Neutral Buffered formalin) do not have a substantial difference. With HT501128, the formaldehyde used for dilution has a formaldehyde content of 37 to 40%. When the product is diluted 1 to 10 with water, it is referred to as 10% formalin, which has a formaldehyde content of 4% formaldehyde. Both products have the same concentration of formaldehyde in solution.

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