MSSAFE
Protease and Phosphatase Inhibitor Cocktail
MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.
Synonym(s):
Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
powder
Solubility:
water: 0.1 g/mL, clear, colorless
Storage temp.:
2-8°C
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Product Name
MS-SAFE Protease and Phosphatase Inhibitor,
description
for use with mammalian cell and tissue extract, lyophilized powder
Quality Level
form
powder
solubility
water: 0.1 g/mL, clear, colorless
storage temp.
2-8°C
Related Categories
1 of 4
This Item | P8465 | P8215 | PIC0007 |
|---|---|---|---|
| form powder | form lyophilized powder | form DMSO solution | form ready-to-use solution |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
| solubility water: 0.1 g/mL, clear, colorless | solubility - | solubility - | solubility - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| description for use with mammalian cell and tissue extract, lyophilized powder | description - | description - | description - |
General description
Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:
- Serine, cysteine and aspartic proteases, and metalloproteases
- Tyrosine, serine/threonine, acid and alkaline phosphatases
Application
MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.[1]
Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.
Biochem/physiol Actions
Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases
Features and Benefits
Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis
Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment
Allows accurate measurement of protein activity and identification of phosphorylation sites.
Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment
Allows accurate measurement of protein activity and identification of phosphorylation sites.
Physical form
Lyophilized powder that is water-soluble
Preparation Note
One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.
Other Notes
Protease inhibitors:
- Bestatin hydrochloride
- Leupeptin
- Phosphoramidon disodium salt
- Pepstatin A
- Elastatinal
- Aprotinin
- Nafamostat mesylate
- Antipain
- Okadaic acid
- Sodium fluoride
- Sodium orthovanadate
- Bromotetramisole oxalate
- β-lactose
- DL-leucine
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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