O7252

Orange G

Name: Orange G
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₁₀N₂Na₂O₇S₂

CAS Number:

1936-15-8

Molecular Weight:

452.37

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24897871

EC Number:

217-705-6

MDL number:

MFCD00012457

Colour Index Number:

16230

Beilstein/REAXYS Number:

4120705

Key Documents

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Product Name

Orange G, certified by the BSC

InChI key

HSXUHWZMNJHFRV-QIKYXUGXSA-L

InChI

1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;

SMILES string

[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

orange to very dark orange

solubility

water: 1 mg/mL, clear

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Show Differences

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This Item	O3756	861286	195235
Sigma-Aldrich O7252 Orange G	Sigma-Aldrich O3756 Orange G	Sigma-Aldrich 861286 Orange G	Sigma-Aldrich 195235 Orange II sodium salt
grade certified by the Biological Stain Commission	grade for molecular biology	grade certified by the Biological Stain Commission	grade -
technique(s) microbe id \| staining: suitable	technique(s) nucleic acid detection: suitable	technique(s) microbe id \| staining: suitable	technique(s) -
color orange to very dark orange	color -	color orange to very dark orange, and Red to Very Dark Red and Orange-Red and Red-Orange and Dark Red-Orange	color -
form powder	form powder	form powder	form powder
solubility water: 1 mg/mL, clear	solubility water: 1 mg/mL, clear, orange to red	solubility H₂O: 1 mg/mL	solubility -
application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology

Application

Certified for use in Mallory′s connective tissue stain; Wilson-Ezrin method for pituitary acidophils; Kreyberg′s method for keratin and mucus; as a counterstain following Heidenhain′s iron hematoxylin on paraffin sections; Flemming′s triple stain on paraffin sections of onion root.

Orange G is typically used as a background or cytoplasmic stain for various biological samples including a common application within clincial cytology within Papanicolaou varients.

General description

Orange G (C.l. 16230) is the disodium salt of benzeneazo-2-naphthol-6,8-disulfonic acid, C₁₆H₁₀N₂O₇S₂Na₂. Orange G, also known as wool orange 2G, is a component in several industry applications including cytology, histology, biology, and industrial staining. It is a component of Mallory′s connective tissue stain.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Integrity of chromatin and replicating DNA in nuclei released from fission yeast by semi-automated grinding in liquid nitrogen.

Givens RM

BMC Research Notes, 4, 499-499 (2011)

Complete degradation of Orange G by electrolysis in sub-critical water.

Asli Yuksel et al.

Journal of hazardous materials, 190(1-3), 1058-1062 (2011-03-29)

Complete degradation of azo dye Orange G was studied using a 500 mL continuous flow reactor made of SUS 316 stainless steel. In this system, a titanium reactor wall acted as a cathode and a titanium plate-type electrode was used

Optimization of Orange G dye adsorption by activated carbon of Thespesia populnea pods using response surface methodology.

M Arulkumar et al.

Journal of hazardous materials, 186(1), 827-834 (2011-01-15)

Thespesia populnea is a large tree found in the tropical regions and coastal forests of India. Its pods were used as a raw material for the preparation of activated carbon. The prepared activated carbon was used for the adsorptive removal

Biosorption of an Azo Dye by Aspergillus niger and Trichoderma sp. Fungal Biomasses.

Arumugam Sivasamy et al.

Current microbiology, 62(2), 351-357 (2010-07-21)

Biosorption is an eco-friendly and cost-effective method for treating the dye house effluents. Aspergillus niger and Trichoderma sp. were cultivated in bulk and biomasses used as biosorbents for the biosorption of an azo dye Orange G. Batch biosorption studies were

Photocatalytic degradation of Orange G dye under solar light using nanocrystalline semiconductor metal oxide.

G Thennarasu et al.

Environmental science and pollution research international, 19(7), 2755-2765 (2012-02-23)

The photocatalytic degradation of Orange G (OG) dye has been investigated using synthesised nanocrystalline ZnO as a photocatalyst and sunlight as the irradiation source. The formation of ZnO prepared from its precursor was confirmed through FT-IR and powder X-ray diffraction

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