SRP6053

ProBDNF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonym(s): Abrineurin, BDNF, Brain-derived neurotrophic factor
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• Product Name: ProBDNF human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
• biological source: human
• recombinant: expressed in E. coli
• assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: ~52 kDa
• packaging: pkg of 10 μg
• NCBI accession no.: P23560
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... BDNF(627)

Description

Biochem/physiol Actions

Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons.[1] As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

General description

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors.[1][2] The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1.[3][4]

Other Notes

Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.

Physical form

Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.

Preparation Note

Centrifuge the vial prior to opening.

Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable