70751
BugBuster® Ni-NTA His•Bind® Purification Kit
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PLN 2,710.00
PLN 2,710.00
About This Item
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manufacturer/tradename
Novagen®
storage condition
OK to freeze
shipped in
wet ice
1 of 4
This Item | 70794 | 70666 | GE28-9034-66 |
|---|---|---|---|
| manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Novagen® | manufacturer/tradename Cytiva |
| shipped in wet ice | shipped in - | shipped in ambient | shipped in - |
| storage condition OK to freeze | storage condition OK to freeze | storage condition do not freeze | storage condition - |
General description
Application
Other Notes
•10,000 UBenzonase Nuclease, purity >90%
•10 mlNi-NTA His•Bind Resin
•pkg/4Chromatography Columns
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Certificates of Analysis (COA)
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Articles
This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.
Related Content
Traditionally, protein purification from E. coli consists of four distinct phases: harvest, bacterial cell lysis, lysate clarification and protein purification. Bacterial lysis typically requires several time-consuming, hands-on steps, such as freeze/thaw cycles and sonication. These harsh lysis techniques may negatively impact protein quality and contribute to sample-to-sample variability. To maintain protein activity and integrity, detergent-based lysis buffers are routinely used to avoid mechanical protein extraction methods. Regardless of the lysis method used, centrifugation is traditionally required to pellet unwanted cell debris and permit recovery of the clarified lysate. The final step, purification, is frequently performed using affinity media specific for expressed epitope tags. Agarose-based media have typically been used, either as a slurry in microcentrifuge tubes or packed into gravity-driven or spin columns. While easier to manipulate, columns are greatly affected by lysate consistency and carryover of cell debris, which can lead to clogging of the column frits.
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