This antibody is an IgG isotype. The whole protein was used for immunization and the epitope has not been mapped. This is a polyclonal antibody and can bind to more than one region.
AB144P
Anti-Choline Acetyltransferase Antibody
CHEMICON®, goat polyclonal
Synonym(s):
Choline acetylase, acetyl CoA:choline O-acetyltransferase, choline acetyltransferase, ChAT
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Select a Size
200 μL
PLN 2,220.00
500 μL
PLN 2,810.00
1 ML
PLN 5,140.00
PLN 2,220.00
Estimated to ship onFebruary 02, 2026Details
A recombinant, preservative-free antibody is available for your target. Try ZRB1012
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Product Name
Anti-Choline Acetyltransferase Antibody, Chemicon®, from goat
biological source
goat
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, opossum, guinea pig, rat, mouse, zebrafish (THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).), avian, monkey, chicken
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
chicken ... Chat(395314)
human ... CHAT(1103)
mouse ... Chat(12647)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)
1 of 4
This Item | AB143 | SAB2500236 | MAB305 |
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form serum | antibody form affinity isolated antibody | antibody form ascites fluid |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| biological source goat | biological source rabbit | biological source goat | biological source mouse |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| technique(s) immunocytochemistry: suitable, western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, western blot: suitable | technique(s) indirect ELISA: suitable, western blot: suitable | technique(s) immunohistochemistry: suitable |
General description
70/74 kDa
Choline acetyltransferase (ChAT) has a molecular weight of about 68 kDa. It is synthesized in the parakaryon and is transported to the axon terminals. ChAT belongs to the choline/carnitine acyltransferase family. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. Choline acetyltransferase is found in cholinergic neurons in brain and spinal cord.
Immunogen
Human placental enzyme.
Application
Anti-Choline Acetyltransferase Antibody has been used in:
Immunohistochemistry:
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.
- immunohistochemical staining
- western blotting
- immunocytochemistry
Immunohistochemistry:
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.
Biochem/physiol Actions
Choline acetyltransferase (CHAT) is involved in the synthesis of acetylcholine in neurons. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CHAT catalyzes the transfer of an acetyl group between acetyl-coenzyme A and choline. Mutations in the gene encoding this protein have been associated with congenital neuromuscular disorders.
Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
Physical form
Affinity purified
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.
For short term storage (3-4 weeks) maintain at 2-8°C.
Analysis Note
Control
Human placenta lysates, rat forebrain/rostral hypothalamus.
Human placenta lysates, rat forebrain/rostral hypothalamus.
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
Other Notes
Research Category:
Neuroscience
Research Sub Category:
Neurotransmitters & Receptors
Neuronal & Glial Markers
Neuroscience
Research Sub Category:
Neurotransmitters & Receptors
Neuronal & Glial Markers
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Han Q, et al.
Experimental Neurology, 194-208 (2015)
The human choline acetyltransferase gene encodes two proteins
Grosman DD, et al.
Journal of Neurochemistry (1995)
Choline acetyltransferase structure reveals distribution of mutations that cause motor disorders
Cai Y, et al.
The Embo Journal, 2047-2047 (2004)
Javier Bernácer et al.
PloS one, 2(11), e1174-e1174 (2007-12-15)
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