ABC40

Anti-AIP1/Alix Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Programmed cell death 6-interacting protein, ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 40

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_001158149
• UniProt accession no.: Q9WU78
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Pdcd6Ip(18571)

Description

General description

Programmed cell death 6-interacting protein (UniProt: Q9WU78; also known as ALG-2-interacting protein 1, ALG-2-interacting protein X, E2F1-inducible protein, Eig2) is encoded by the Pdcd6ip (also known as Aip1, Alix) gene (Gene ID: 18571) in murine species. AIP1 is a class E VPS cytoplasmic, ubiquitously expressed protein that is involved in concentration and sorting of cargo proteins of the multi-vesicular body (MVB) for incorporation into intra-lumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. AIP1 interacts with ALG-2 (apoptosis-linked gene-2) to regulate cell death. This interaction is strictly dependent on calcium. AIP1 is considered to be essential for the maintenance of fibroblast morphology and has been shown to play a role in the regulation of both apoptosis and cell proliferation. AIP1 is also known to regulate exosome biogenesis in concert with SDC1/4 and SDCBP. AIP1 can undergo phosphorylated on tyrosine residues by activated platelet-derived growth factor receptor-beta (PDGFRB).

~95 kDa observed; 96.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

GST-tagged recombinant fragment corresponding to 479 amino acids from the C-terminal half of mouse AIP1/Alix.

Application

Detect Programmed cell death 6-interacting protein using this rabbit polyclonal Anti-AIP1/Alix, Cat. No. ABC40 that has been tested in Immunocytochemistry and Western blotting.

Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected AIP1/Alix in HeLa and NIH3T3 cells.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This polyclonal antibody specifically detects AIP1 in Jurkat cells. It is likely to react with isoforms 1, 2, and 3.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 20 mM Tris-HCl, pH 7.2, 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected AIP1/Alix in 10 µg of Jurkat cell lysate.

Control
HeLa cell lysate

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2