APT129

Caspase 8 Colorimetric Activity Assay Kit 25, IETD

Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD.

• Synonym(s): FLICE
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 100
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Chemicon^®
• technique(s): activity assay: suitable
• NCBI accession no.: NM_001080124.1; NM_001080125.1; NM_001228.4; NM_033355.3; NM_033356.3; NM_033358.3
• UniProt accession no.: Q14790
• detection method: colorimetric
• shipped in: dry ice

Description

General description

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon′s Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

Application

Research Category
Apoptosis & Cancer

Components

5X Cell Lysis Buffer (Part No. 90065): 5 mL

5X Assay Buffer (Part No. 90066): 10 mL

Caspase-8 Substrate (Ac-IETD-pNA) (Part No. 90081): 250 μL of 2.5 mg/mL solution

Caspase-8 Inhibitor (Ac-IETD-CHO) (Part No. 90086): 50 μL of 100 mM (0.05 mg/mL) in DMSO

pNA Standard (Part No. 90085): 250 μL of 10 mM in DMSO

Storage and Stability

Store kit materials at -20°C up to their expiration date.

Special Precautions:

· After thawing reagents, use immediately or aliquot and freeze at -20oC for longer storage. Avoid repeated freeze/thaw cycles.

· The Caspase-8 substrate and pNA standard are especially light sensitive. Maintain these reagents in amber or covered containers.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2