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Anti-ACTN4 Mouse mAb (4D10)

Name: Anti-ACTN4 Mouse mAb (4D10)
Brand: MM

liquid, clone 4D10, Calbiochem^®

Synonym(s):

Anti-Actinin α4

About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

4D10, monoclonal

Species reactivity:

mouse, human, rat

Application:

—

Citations:

This product is discontinued. Contact Technical Service for assistance.

Properties

biological source

mouse

Quality Segment

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4D10, monoclonal

form

liquid

does not contain

preservative

species reactivity

mouse, human, rat

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (20 µg/mL)
Paraffin Sections (3 µg/mL))

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTN4(81)

Description

General description

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.

Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.

Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (20 g/ml)
Paraffin Sections (3 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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This Item	WH0000081M1	HPA001873	SAB5600102
Sigma-Aldrich CB1024 Anti-ACTN4 Mouse mAb (4D10)	Sigma-Aldrich WH0000081M1 Monoclonal Anti-ACTN4 antibody produced in mouse	Sigma-Aldrich HPA001873 Anti-ACTN4 antibody produced in rabbit	Sigma-Aldrich SAB5600102 Anti-Alpha Actinin-4 antibody, Rabbit Monoclonal
description liquid, clone 4D10, Calbiochem^®	description clone 4D10, purified immunoglobulin, buffered aqueous solution	description Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution	description recombinant, expressed in HEK 293 cells, clone RM287, purified immunoglobulin
biological source mouse	biological source mouse	biological source rabbit	biological source -
clone 4D10, monoclonal	clone 4D10, monoclonal	clone polyclonal	clone RM287, monoclonal, recombinant monoclonal
species reactivity mouse, human, rat	species reactivity mouse, rat, human	species reactivity mouse, human, rat	species reactivity human
antibody form purified antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin
Gene Information human ... ACTN4(81)	Gene Information human ... ACTN4(81)	Gene Information human ... ACTN4(81)	Gene Information human ... ACTN4(81)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

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