CBA059

Annexin V-FITC Apoptosis Detection Kit II

fluorometric assay kit for measuring Annexin V in apoptotic cells.

Properties

• Quality Level: 100
• usage: sufficient for 20 tests
• packaging: pkg of 1 kit
• manufacturer/tradename: Calbiochem^®
• technique(s): flow cytometry: suitable; immunofluorescence: suitable
• detection method: fluorometric
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

A convenient kit for the identification of changes in the plasma membrane that occur during apoptosis. In apoptotic cells, phosphoatidylserine (PS) is translocated from the inner to the outer leaflet of the plasma membrane, allowing for the detection of PS on the cell surface. In the presence of Ca 2⁺, Annexin V exhibits a high affinity for PS and binds cells with exposed PS. Annexin V, when conjugated to a fluorophore, can be used to monitor apoptopic cells using flow cytometry or immunofluorescence microscopy.

Components

Kit contains:

• Annexin V-FITC
• Binding Buffer
• Propidium Iodide
• user protocol.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2