IM40

Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2)

liquid, clone 141-7B2, Calbiochem^®

• Synonym(s): Anti-Matrilysin, Anti-Matrix Metalloproteinase 7, Anti-PUMP-1
• NACRES: NA.41
• UNSPSC Code: 12352203

Properties

• Product Name: Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2), liquid, clone 141-7B2, Calbiochem^®
• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 141-7B2, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Immunoblotting (5-10 µg/mL, ; Paraffin Sections )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... MMP7(4316)

Description

Analysis Note

Positive Control
CaR-1 or TPA treated SW620 cells

Application

Immunoblotting (5-10 g/ml, see application references)
Paraffin Sections (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Purified mouse monoclonal antibody. Recognizes the ~28 kDa latent form of MMP-7.

Recognizes the ~28 kDa latent form of MMP-7 in TPA-treated SW620 cells. Does not recognize the active form of MMP-7.

This Anti-MMP-7 (Ab-1) Mouse mAb (141-7B2) is validated for use in Immunoblotting, Paraffin Sections for the detection of MMP-7 (Ab-1).

Immunogen

Human

human rectal carcinoma MMP-7

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Brunner, K.L, et al. 1995. Proc. Natl. Acad. Sci. USA92, 7362.
Imai, K., et al. 1995. J. Biol. Chem.270, 6691.
Lichtinghagen, R., et al. 1995. Eur. J. Clin. Chem. Clin. Biochem.33, 65.
Nakano, A., et al. 1995. J. Neurosurg.83, 298.
Takino, T., et al. 1995. J. Biol. Chem.270, 23013.
Woessner, J.F. 1991. FASEB J.5, 2145.
Yamamoto, H., et al. 1995. J. Clin. Lab. Anal.9, 297.
Gaire, M., et al. 1994. J. Biol. Chem.269, 2032.
McDonnell, S., et al. 1994. Biochem. Soc. Trans.22, 58.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M. M. Gottesman. Vol. 1(2), 99.
Woessner, J.F., Jr and Taplin, C.J. 1988. J. Biol. Chem.263, 16918.
Sellers, A. and Woessner, J.F., Jr. 1980. Biochem. J.189, 521.

Does not recognize the ~19 kDa active form of MMP-7. To detect active MMP-7, use Cat. No. IM47L. Does not cross-react with human MMP-1, MMP-2, MMP-3, MMP-8, MMP-9 or MMP-13. For optimal detection, SW620 cells should be treated with TPA (100 ng/ml) to induce expression of MMP-7. Antibody should be titrated for optimal results in individual systems.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable