IP05

Protein G Plus/Protein A Agarose Suspension

Name: Protein G Plus/Protein A Agarose Suspension
Brand: MM
Price: 757 PLN
Availability: InStock

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Synonym(s):

Affinity resin, Protein G/Agarose

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1.5 ML

PLN 757.00

Estimated to ship TODAYDetails

About This Item

UNSPSC Code:

41116133

NACRES:

NA.56

Key Documents

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

technique(s)

protein purification: suitable

suitability

suitable for microbiology

shipped in

wet ice

storage temp.

2-8°C

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This Item	IP04	IP10	P1925
Millipore IP05 Protein G Plus/Protein A Agarose Suspension	Millipore IP04 Protein G Plus-Agarose Suspension	Millipore IP10 Protein G Plus/Protein A-Agarose	Millipore P1925 Protein A–Agarose macrobeads
technique(s) protein purification: suitable	technique(s) immunoprecipitation (IP): suitable	technique(s) protein purification: suitable	technique(s) -
form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)	form aqueous suspension
suitability suitable for microbiology	suitability suitable for immunoprecipitation	suitability suitable for microbiology	suitability -
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains -
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.

Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.

Application

Immunoprecipitation (see comments)

Physical form

33% slurry in PBS.

Other Notes

Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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SUMOylation of Grb2 enhances the ERK activity by increasing its binding with Sos1.

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The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify

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Protein G Plus/Protein A Agarose Suspension