Merck
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IPFL07810

Millipore

Immobilon®-FL PVDF Transfer Membrane

0.45 μm pore size, 7 X 8.4 cm sheets

Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602
NACRES:
NB.22

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin)
205 μg/cm2 adsorption capacity (BSA)
300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Coomassie brilliant blue
for use with Ponceau-S red

detection method

chemiluminescent
colorimetric
fluorometric

shipped in

ambient

Related Categories

General description

The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and is recommended for blotting proteins with molecular weights greater than 20 kilodaltons (kDa). The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection.
Filter Code: IPFL

Application

Immobilon®-FL Transfer Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Transfer Membrane:
  • Designed for optimal fluorescent immunodetection application
  • Extremely low background improves the sensitivity of all fluorescence based immunodetection
  • Compatible with all commonly used fluorescent probes across a wide range of excitation/emission wavelengths
  • For standard chemiluminescent or chromogenic detection

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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