Merck
  • MABC006
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MABC006

Sigma-Aldrich

Mouse IgG2b Negative Control, clone GC198

Mouse IgG2b Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

eCl@ss:
32160702
NACRES:
NA.42

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

GC198, monoclonal

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG2b

shipped in

wet ice

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biological source

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antibody form

purified immunoglobulin

antibody form

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antibody form

affinity isolated antibody

antibody form

purified antibody

clone

GC198, monoclonal

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manufacturer/tradename

-

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

0.5 mg/mL

concentration

-

technique(s)

flow cytometry: suitable, immunofluorescence: suitable

technique(s)

flow cytometry: suitable, immunofluorescence: suitable

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable

Specificity

This antibody reacts with the outer membrane protein of some strains of Neisseria gonorrhoeae. No reaction with human cell surface or plasma components has been observed. However, some binding to Fc receptors may occur.

Immunogen

Protein I preparation from Neisseria gonorrhoeae.

Application

Mouse IgG2b is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Linkage

Replaces: CBL602

Physical form

Format: Purified
Purified immunoglobulin. The antibody is supplied in 0.5ml 25mM Tris-HCl, 0.4M NaCl, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis, specific immunofluorescence assay and flow cytometry.

Storage and Stability

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Please enter the Product Number and Lot Number.

Certificate of Quality

Please enter the Product Number and Lot Number.

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