MABF736

Anti-Ly-6G Antibody (mouse), APC, clone 1A8

clone 1A8, 0.2 mg/mL, from rat

• Synonym(s): Lymphocyte antigen 6G, Ly-6G, Ly-6G.1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: Allophycocyanin conjugate
• Clone: 1A8, monoclonal
• Application: FACS
• Citations: -

Properties

• biological source: rat
• Quality Segment: 100
• conjugate: Allophycocyanin conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1A8, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• concentration: 0.2 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG2aκ
• UniProt accession no.: P35461
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ly6G(546644)

Description

General description

The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the 1A8 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes. Note: for identification of Ly-C, an alternative antibody, clone RB6-8C5, has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This rat monoclonal antibody Anti-Ly-6G (mouse), APC & clone 1A8 validated for use in Flow Cytometry for the detection of Ly-6G.

Physical form

Purified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg of this antibody detected Ly-6G in one million C57Bl/6 bone marrow cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg