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WBLUC

Immobilon® Classico Western HRP substrate

Western blotting, dot/slot blotting

Synonym(s):

HRP substrate, chemiluminescent detection substrate, detection substrate

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WBLUC0100

PLN 447.00

WBLUC0500

PLN 1,280.00

PLN 447.00

Estimated to ship onDecember 04, 2025Details

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About This Item

UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22

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Product Name

Immobilon Classico Western HRP substrate, Western blotting, dot/slot blotting

conjugate

peroxidase conjugate

Quality Level

200

manufacturer/tradename

Luminata

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

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Immobilon Classico Western HRP substrate Western blotting, dot/slot blotting

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Immobilon Crescendo Western HRP substrate, 100 mL

Immobilon Western Chemiluminescent HRP Substrate Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.

Millipore

WBKLS

Immobilon Western Chemiluminescent HRP Substrate

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

detection method

chemiluminescent

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

manufacturer/tradename

Luminata

manufacturer/tradename

Immobilon®

manufacturer/tradename

Luminata

manufacturer/tradename

Immobilon®

General description

Premixed, ready to use chemiluminescent HRP detection reagent

Application

For use on immunoblots.
Western blotting, dot/slot blotting

Packaging

1 bottle for 1000 cm2

Preparation Note

Stable for 1 year at 4°C from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.

Analysis Note

The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.

Legal Information

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Implication of RPA32 phosphorylation in S-phase checkpoint signalling at replication forks stalled with aphidicolin in Xenopus egg extracts.
Recolin, Benedicte and Maiorano, Domenico
Biochemical and biophysical research communications (2012)

Articles

Western Blotting: Why Are Observed and Calculated Molecular Weights Different?

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protocols

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

Wprowadzenie do wykrywania białek metodą Western Blot

Western blotting obejmuje rozdzielanie białek za pomocą elektroforezy żelowej, przenoszenie białek na membranę z polifluorku winylidenu (PVDF) lub nitrocelulozy oraz wykrywanie różnymi metodami.

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